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SELECT
id,
product_name,
product_catalog_number,
supplier_id,
supplier_name,
product_detail_size,
product_detail_price_buy,
product_detail_price_sell,
product_detail_description,
product_detail_specifications,
product_detail_additional_information,
product_detail_storage_and_shipping,
product_detail_notes,
product_type,
main_image
FROM gentaur_products
WHERE id IN ("Cusabio|1-CSB-PA018886GA01HU","Cusabio|1-CSB-PA018886LA01HU","Cusabio|1-CSB-PA775839","SAB|22848-100ul","SAB|43134-100ul","Lifescience Market|ELI-15652r","St John\'s Laboratory|STJ29479","St John\'s Laboratory|STJ11100809","SAB|C43134","Lifescience Market|PVT18497","Abbexa|20-abx930156","Abbexa|20-abx930157","Abbexa|20-abx904317","Cusabio|1-CSB-PA018886LB01HU","Cusabio|1-CSB-PA018886LC01HU","Cusabio|1-CSB-PA018886LD01HU","Affbiotech|BF0434-BP","Abfrontier|LF-MA30618","Abclonal|A7340-100ul","Abclonal|A7340-200ul","Abclonal|A7340-20ul","Abclonal|A7340-50ul","Leading Biology|AMM02864G","BosterBio|M02188-2","Fitzgerald|80R-2608","Lifescience Market|ELI-15651d","ABM|RP085356","ABM|RP165287","ABM|RP222629","Abbexa|20-abx984734","Abbexa|20-abx953843","Abbexa|20-abx971337","ABM|ORF074211","ABM|ORF028453","ABM|ORF055097","Abclonal|A19933-100ul","Abclonal|A19933-200ul","Abclonal|A19933-20ul","Abclonal|A19933-50ul","Aviva Systems Biology|OKEH03975","Aviva Systems Biology|OKCD02695","Aviva Systems Biology|OKEH07770","Abbexa|20-abx114776","Abbexa|20-abx211178","Abbexa|20-abx211851","Abbexa|20-abx141396","Abbexa|20-abx006453","Abbexa|abx011689-100ul","Abbexa|abx028154-400ul","Abbexa|abx028154-80l","Leading Biology|AMM03954G","ABM|K6921501","ABM|K4425601","ABM|K1740301","ABM|TU019046","ABM|TU117173","ABM|TU167173","ABM|TU216964","ABM|TU266964","ABM|TU069046","ABM|PV113810","ABM|PV113811","ABM|PV113812","ABM|PV113813","ABM|PV296842","ABM|PV296843","ABM|PV296844","ABM|PV296845","ABM|PV220386","ABM|PV220387","ABM|PV220388","ABM|PV220389","Cusabio|1-CSB-EP018886HU","ABM|K6921502","ABM|K6921503","ABM|K6921504","EnQuireBio|QP13190-1mg","EnQuireBio|QP13190-20ug","EnQuireBio|QP13190-5ug","ABM|K4425602");
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WordPress database error: [Unknown column 'main_image' in 'field list']
SELECT
id,
product_name,
product_catalog_number,
supplier_id,
supplier_name,
product_detail_size,
product_detail_price_buy,
product_detail_price_sell,
product_detail_description,
product_detail_specifications,
product_detail_additional_information,
product_detail_storage_and_shipping,
product_detail_notes,
product_type,
main_image
FROM gentaur_products
WHERE id IN ("CHI Scientific|7-03631","CHI Scientific|7-03632","CHI Scientific|7-03633","ABM|G008","ABM|G009","ABM|G126","Vazyme|P101-01","Vazyme|P101-02","Vazyme|P101-03","Bio Basic|D0090","Alpha Diagnostics|RP-395","GeneOn|N140","GeneOn|N142","GeneOn|N144","Biovision|9001-2500","Biovision|9001-500","Biochain|L7051001","Biochain|L7051200","Cusabio|BA00103","Bioatlas|BA00104","Bioatlas|BA00105","Bioatlas|BT10101","Bioatlas|BT10102","Bioatlas|BT10103","Vazyme|P101-d1","Vazyme|P101-d2","Vazyme|P101-d3","Vazyme|P122-d1","Vazyme|P122-d2","Vazyme|P122-d3","Biochain|L7052001","Biochain|L7052250","Bio Basic|9K-001-0001","Bio Basic|9K-001-0002","Bio Basic|9K-001-0033","Bio Basic|9K-001-0035","Abbexa|abx073521-10000U","Abbexa|abx073521-1000U","Abbexa|abx073521-3000U","CHI Scientific|7-03634","CHI Scientific|7-03635","CHI Scientific|7-03636","Sceti|02-001","Sceti|02-001-5","Sceti|02-011","Sceti|02-011-5","GeneOn|S101","GeneOn|S102","GeneOn|S103","GenDepot|T2200-050","GenDepot|T2200-100","GenDepot|T2200-200","ABM|G014","ABM|G901","Vazyme|P201-d1","Vazyme|P201-d2","Vazyme|P201-d3","Biovision|7018-100","Biovision|7018-30T","Bio Basic|9K-001-0036","Bio Basic|B0089(D0089)","Intact Genomics|3245","Yeastern Biotech|FYT011-500U","ACTGene|E2100-1000U","ACTGene|E2100-250U","SAB|48369-100ul","SAB|48369-50ul","Alpha Diagnostics|RP-394","Abbexa|abx071022-100U","Abbexa|abx071022-1200U","Abbexa|abx071022-200U","Alpha Diagnostics|AD-146-B","Alpha Diagnostics|AD-146-U","BenchMark|PR1000-TP-250","BenchMark|PR1000-TP-500","BenchMark|PR1000-TP-S","Vazyme|P102-01","Vazyme|P102-02","Vazyme|P102-03","Biotium|29050","Yeastern Biotech|FYT001-500U","GenDepot|P0322-010","GenDepot|P0322-025","ACTGene|E2100B-1000U","ACTGene|E2100B-250U","GenDepot|T2200-B01","GenDepot|T2200-B05","GenDepot|T2201-050","GenDepot|T2201-100","GenDepot|T2201-200","Allele Biotech|ABP-PP-ERTQ001","Allele Biotech|ABP-PP-ERTQ100","Allele Biotech|ABP-PP-ERTQ200","Allele Biotech|ABP-PP-ERTQ500");
Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays.
SARS-CoV-2 enters cells utilizing its Spike protein, which can also be the primary goal of neutralizing antibodies. Due to this fact, assays to measure how antibodies and sera have an effect on Spike-mediated viral an infection are vital for finding out immunity. As a result of SARS-CoV-2 is a biosafety-level-Three virus, one option to simplify such assays is to pseudotype biosafety-level-2 viral particles with Spike. Such pseudotyping has now been described for single-cycle lentiviral, retroviral, and vesicular stomatitis virus (VSV) particles, however the reagents and protocols will not be extensively out there.
Right here, we detailed tips on how to successfully pseudotype lentiviral particles with SARS-CoV-2 Spike and infect 293T cells engineered to precise the SARS-CoV-2 receptor, ACE2. We additionally made all the important thing experimental reagents out there within the BEI Assets repository of ATCC and the NIH. Moreover, we demonstrated how these pseudotyped lentiviral particles could possibly be used to measure the neutralizing exercise of human sera or plasma in opposition to SARS-CoV-2 in handy luciferase-based assays, thereby offering a invaluable complement to ELISA-based strategies that measure antibody binding moderately than neutralization.
gentaur.com
 PSMB8 Antibody |
|
1-CSB-PA018886GA01HU |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF |
PSMB8 Antibody |
|
1-CSB-PA018886LA01HU |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
PSMB8 Antibody |
|
1-CSB-PA775839 |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100 |
 PSMB8 antibody |
|
22848-100ul |
SAB |
100ul |
EUR 390 |
PSMB8 Antibody |
|
43134-100ul |
SAB |
100ul |
EUR 252 |
 Anti-PSMB8 antibody |
|
STJ29479 |
St John's Laboratory |
100 µl |
EUR 277 |
|
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. |
Anti-PSMB8 antibody |
|
STJ11100809 |
St John's Laboratory |
100 µl |
EUR 413 |
|
Description: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. |
 PSMB8 Conjugated Antibody |
|
C43134 |
SAB |
100ul |
EUR 397 |
 PSMB8 siRNA |
|
20-abx930156 |
Abbexa |
|
|
|
|
PSMB8 siRNA |
|
20-abx930157 |
Abbexa |
|
|
|
|
PSMB8 siRNA |
|
20-abx904317 |
Abbexa |
|
|
|
|
 PSMB8 Antibody, HRP conjugated |
|
1-CSB-PA018886LB01HU |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
 PSMB8 Antibody, FITC conjugated |
|
1-CSB-PA018886LC01HU |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
 PSMB8 Antibody, Biotin conjugated |
|
1-CSB-PA018886LD01HU |
Cusabio |
|
|
|
|
|
Description: A polyclonal antibody against PSMB8. Recognizes PSMB8 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
 PSMB8 Blocking Peptide |
|
BF0434-BP |
Affbiotech |
1mg |
EUR 195 |
) anti-PSMB8 (1A5) |
|
LF-MA30618 |
Abfrontier |
100 ul |
EUR 527 |
|
Description: Mouse Monoclonal to PSMB8 |
 PSMB8 Rabbit pAb |
|
A7340-100ul |
Abclonal |
100 ul |
EUR 308 |
PSMB8 Rabbit pAb |
|
A7340-200ul |
Abclonal |
200 ul |
EUR 459 |
PSMB8 Rabbit pAb |
|
A7340-20ul |
Abclonal |
20 ul |
EUR 183 |
PSMB8 Rabbit pAb |
|
A7340-50ul |
Abclonal |
50 ul |
EUR 223 |
 Monoclonal PSMB8 Antibody, Clone: 1A5 |
|
AMM02864G |
Leading Biology |
0.1ml |
EUR 528 |
|
Description: A Monoclonal antibody against Human PSMB8. The antibodies are raised in Mouse and are from clone 1A5. This antibody is applicable in WB and IHC, ICC, E |
 Anti-PSMB8 / LMP7 Monoclonal Antibody |
|
M02188-2 |
BosterBio |
100ug |
EUR 397 |
|
Description: Rabbit Monoclonal PSMB8 / LMP7 Antibody. Validated in IP, IF, WB and tested in Human, Mouse, Rat. |
) PSMB8 protein (His tag) |
|
80R-2608 |
Fitzgerald |
100 ug |
EUR 322 |
|
Description: Purified recombinant PSMB8 protein |
) PSMB8 Recombinant Protein (Human) |
|
RP085356 |
ABM |
100 ug |
Ask for price |
) PSMB8 Recombinant Protein (Mouse) |
|
RP165287 |
ABM |
100 ug |
Ask for price |
) PSMB8 Recombinant Protein (Rat) |
|
RP222629 |
ABM |
100 ug |
Ask for price |
 Rat PSMB8 shRNA Plasmid |
|
20-abx984734 |
Abbexa |
|
|
|
|
 Human PSMB8 shRNA Plasmid |
|
20-abx953843 |
Abbexa |
|
|
|
|
 Mouse PSMB8 shRNA Plasmid |
|
20-abx971337 |
Abbexa |
|
|
|
|
 (pORF)) Psmb8 ORF Vector (Rat) (pORF) |
|
ORF074211 |
ABM |
1.0 ug DNA |
EUR 506 |
 (pORF)) PSMB8 ORF Vector (Human) (pORF) |
|
ORF028453 |
ABM |
1.0 ug DNA |
EUR 405 |
 (pORF)) Psmb8 ORF Vector (Mouse) (pORF) |
|
ORF055097 |
ABM |
1.0 ug DNA |
EUR 506 |
 [KO Validated] PSMB8 Rabbit pAb |
|
A19933-100ul |
Abclonal |
100 ul |
EUR 410 |
[KO Validated] PSMB8 Rabbit pAb |
|
A19933-200ul |
Abclonal |
200 ul |
EUR 571 |
[KO Validated] PSMB8 Rabbit pAb |
|
A19933-20ul |
Abclonal |
20 ul |
EUR 221 |
[KO Validated] PSMB8 Rabbit pAb |
|
A19933-50ul |
Abclonal |
50 ul |
EUR 287 |
 (OKEH03975)) PSMB8 ELISA Kit (Dog) (OKEH03975) |
|
OKEH03975 |
Aviva Systems Biology |
96 Wells |
EUR 844 |
|
Description: Description of target: ;Species reactivity: Dog;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.064 ng/mL |
 (OKCD02695)) PSMB8 ELISA Kit (Human) (OKCD02695) |
|
OKCD02695 |
Aviva Systems Biology |
96 Wells |
EUR 792 |
|
Description: Description of target: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit is involved in antigen processing to generate class I binding peptides. Replacement of PSMB5 by PSMB8 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. Acts as a major component of interferon gamma-induced sensitivity. Plays a key role in apoptosis via the degradation of the apoptotic inhibitor MCL1. May be involved in the inflammatory response pathway. In cancer cells, substitution of isoform 1 (E2) by isoform 2 (E1) results in immunoproteasome deficiency. Required for the differentiation of preadipocytes into adipocytes.;Species reactivity: Human;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.117 ng/mL |
 (OKEH07770)) PSMB8 ELISA Kit (Human) (OKEH07770) |
|
OKEH07770 |
Aviva Systems Biology |
96 Wells |
EUR 1092 |
|
Description: Description of target: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.06ng/mL |
 Antibody) Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
20-abx114776 |
Abbexa |
|
|
|
|
 Antibody) Proteasome subunit beta type-8 (PSMB8) Antibody |
|
20-abx211178 |
Abbexa |
|
|
|
|
Proteasome subunit beta type-8 (PSMB8) Antibody |
|
20-abx211851 |
Abbexa |
|
|
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
20-abx141396 |
Abbexa |
-
EUR 370.00
-
EUR 606.00
-
EUR 300.00
|
|
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
20-abx006453 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
-
100 ul
-
200 ul
-
20 ul
-
50 ul
|
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
abx011689-100ul |
Abbexa |
100 ul |
EUR 411 |
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
abx028154-400ul |
Abbexa |
400 ul |
EUR 523 |
|
|
Proteasome Subunit Beta Type-8 (PSMB8) Antibody |
|
abx028154-80l |
Abbexa |
80 µl |
EUR 286 |
|
|
 (M01), Clone: 1B3) Monoclonal PSMB8 Antibody (monoclonal) (M01), Clone: 1B3 |
|
AMM03954G |
Leading Biology |
0.1mg |
EUR 484 |
|
Description: A Monoclonal antibody against Human PSMB8 (monoclonal) (M01). The antibodies are raised in Mouse and are from clone 1B3. This antibody is applicable in WB, IHC and IF, IP, E |
) Psmb8 sgRNA CRISPR Lentivector set (Rat) |
|
K6921501 |
ABM |
3 x 1.0 ug |
EUR 339 |
) Psmb8 sgRNA CRISPR Lentivector set (Mouse) |
|
K4425601 |
ABM |
3 x 1.0 ug |
EUR 339 |
) PSMB8 sgRNA CRISPR Lentivector set (Human) |
|
K1740301 |
ABM |
3 x 1.0 ug |
EUR 339 |
 PSMB8 3'UTR Luciferase Stable Cell Line |
|
TU019046 |
ABM |
1.0 ml |
EUR 1394 |
 Psmb8 3'UTR Luciferase Stable Cell Line |
|
TU117173 |
ABM |
1.0 ml |
Ask for price |
 Psmb8 3'UTR GFP Stable Cell Line |
|
TU167173 |
ABM |
1.0 ml |
Ask for price |
Psmb8 3'UTR Luciferase Stable Cell Line |
|
TU216964 |
ABM |
1.0 ml |
Ask for price |
Psmb8 3'UTR GFP Stable Cell Line |
|
TU266964 |
ABM |
1.0 ml |
Ask for price |
 PSMB8 3'UTR GFP Stable Cell Line |
|
TU069046 |
ABM |
1.0 ml |
EUR 1394 |
 (pPB-C-His)) PSMB8 Protein Vector (Human) (pPB-C-His) |
|
PV113810 |
ABM |
500 ng |
EUR 552 |
 (pPB-N-His)) PSMB8 Protein Vector (Human) (pPB-N-His) |
|
PV113811 |
ABM |
500 ng |
EUR 552 |
 (pPM-C-HA)) PSMB8 Protein Vector (Human) (pPM-C-HA) |
|
PV113812 |
ABM |
500 ng |
EUR 552 |
 (pPM-C-His)) PSMB8 Protein Vector (Human) (pPM-C-His) |
|
PV113813 |
ABM |
500 ng |
EUR 552 |
 (pPB-C-His)) PSMB8 Protein Vector (Rat) (pPB-C-His) |
|
PV296842 |
ABM |
500 ng |
EUR 603 |
 (pPB-N-His)) PSMB8 Protein Vector (Rat) (pPB-N-His) |
|
PV296843 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-HA)) PSMB8 Protein Vector (Rat) (pPM-C-HA) |
|
PV296844 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-His)) PSMB8 Protein Vector (Rat) (pPM-C-His) |
|
PV296845 |
ABM |
500 ng |
EUR 603 |
 (pPB-C-His)) PSMB8 Protein Vector (Mouse) (pPB-C-His) |
|
PV220386 |
ABM |
500 ng |
EUR 603 |
 (pPB-N-His)) PSMB8 Protein Vector (Mouse) (pPB-N-His) |
|
PV220387 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-HA)) PSMB8 Protein Vector (Mouse) (pPM-C-HA) |
|
PV220388 |
ABM |
500 ng |
EUR 603 |
 (pPM-C-His)) PSMB8 Protein Vector (Mouse) (pPM-C-His) |
|
PV220389 |
ABM |
500 ng |
EUR 603 |
) Human Proteasome subunit beta type-8 (PSMB8) |
|
1-CSB-EP018886HU |
Cusabio |
-
EUR 380.00
-
EUR 214.00
-
EUR 1309.00
-
EUR 560.00
-
EUR 873.00
-
EUR 262.00
|
-
100ug
-
10ug
-
1MG
-
200ug
-
500ug
-
50ug
|
|
|
|
Description: Recombinant Human Proteasome subunit beta type-8(PSMB8) expressed in E.coli |
 (Target 1)) Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 1) |
|
K6921502 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 2)) Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 2) |
|
K6921503 |
ABM |
1.0 ug DNA |
EUR 154 |
 (Target 3)) Psmb8 sgRNA CRISPR Lentivector (Rat) (Target 3) |
|
K6921504 |
ABM |
1.0 ug DNA |
EUR 154 |
 Recombinant Human PSMB8 Protein, His, E.coli-1mg |
|
QP13190-1mg |
EnQuireBio |
1mg |
EUR 2757 |
 Recombinant Human PSMB8 Protein, His, E.coli-20ug |
|
QP13190-20ug |
EnQuireBio |
20ug |
EUR 201 |
 Recombinant Human PSMB8 Protein, His, E.coli-5ug |
|
QP13190-5ug |
EnQuireBio |
5ug |
EUR 155 |
 (Target 1)) Psmb8 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
|
K4425602 |
ABM |
1.0 ug DNA |
EUR 154 |
Comparative analysis of nucleic acid stabilizing reagents for RNA- and DNA-based Leishmania detection in blood as proxy for visceral burdens.
Molecular detection methods utilizing peripheral blood are most popular over invasive tissue aspiration for the prognosis and post-treatment follow-up of visceral leishmaniasis (VL) sufferers. This research goals to determine appropriate stabilizing reagents to stop DNA and RNA degradation throughout storage and transport to specialised laboratories the place molecular prognosis is carried out.
- The stabilizing capacities of various commercially out there reagents had been in contrast utilizing promastigote-spiked human blood and peripheral blood of Syrian golden hamsters subjected to experimental an infection, therapy (miltefosine of aminopyrazole DNDi-1044) and immunosuppression.
- The influence of assorted storage temperature circumstances was examined together with a longtime kinetoplast DNA (kDNA) qPCR and a lately developed spliced chief RNA (SL-RNA) assay for Leishmania detection.
- Regardless of the blood sort and stabilizer used, threshold (cT) values obtained with the SL-RNA qPCR had been systematically decrease than these obtained with the kDNA assay, confirming the benefit of the SL-RNA assay over the extensively used kDNA assay for low-level Leishmania detection. Peripheral blood parasite ranges correlated comparatively properly with hepatic burdens.
- RNA Shield Cell Reagent supplied essentially the most optimum simultaneous DNA and RNA stabilization in each human and hamster blood. Nevertheless, this stabilizer requires an erythrocyte lysis step, which may be difficult underneath discipline circumstances.
- DNA/RNA Protect gives a very good various for downstream kDNA and SL-RNA assays, particularly if pattern storage capability at 4 °C may be assured.
- The advisable stabilizing reagents are appropriate with RNA- and DNA-based Leishmania detection in peripheral blood within the VL hamster mannequin and spiked human blood.
- Since molecular detection methods utilizing peripheral blood are much less invasive than microscopic evaluation of tissue aspirates, the findings of this research could also be utilized to human VL medical research.
Conjugate Addition of Grignard Reagents to Thiochromones Catalyzed by Copper Salts: A Unified Method to Each 2-Alkylthiochroman-4-One and Thioflavanone.
Grignard reagents endure conjugate addition to thiochromones catalyzed by copper salts to afford 2-substituted-thiochroman-4-ones, each 2-alkylthiochroman-4-ones and thioflavanones (2-arylthiochroman-4-ones), in good yields with trimethylsilyl chloride (TMSCl) as an additive.
- The very best yields of 1,4-adducts may be attained with CuCN∙2LiCl because the copper supply. Glorious yields of 2-alkyl-substituted thiochroman-4-ones and thioflavanones (2-aryl substituted) are attained with a broad vary of Grignard reagents.
- This strategy works properly with each alkyl and fragrant Grignard reagents, thus offering a unified artificial strategy to privileged 2-substituted thiochroman-4-ones and a possible invaluable precursor for additional artificial purposes in direction of many pharmaceutically lively molecules.
- Using commercially out there and/or readily ready Grignard reagents will expedite the synthesis of a giant library of each 2-alkyl substituted thiochroman-4-ones and thioflavanones for extra artificial purposes.
 Recombinant Taq Plus DNA Polymerase |
|
7-03631 |
CHI Scientific |
250U |
Ask for price |
Recombinant Taq Plus DNA Polymerase |
|
7-03632 |
CHI Scientific |
750U |
Ask for price |
Recombinant Taq Plus DNA Polymerase |
|
7-03633 |
CHI Scientific |
2,500U |
Ask for price |
) Taq DNA Polymerase (PCR) |
|
G008 |
ABM |
5000 U (1.0 ml) |
EUR 205 |
Taq DNA Polymerase (PCR) |
|
G009 |
ABM |
1000 U (200 ul) |
EUR 87 |
Taq DNA Polymerase (PCR) |
|
G126 |
ABM |
10000 U (2 x 1.0 ml) |
EUR 309 |
) Taq DNA Polymerase (Mg2+ plus Buffer) |
|
P101-01 |
Vazyme |
1000 U |
EUR 121 |
Taq DNA Polymerase (Mg2+ plus Buffer) |
|
P101-02 |
Vazyme |
5000 U |
EUR 193 |
Taq DNA Polymerase (Mg2+ plus Buffer) |
|
P101-03 |
Vazyme |
10000 U |
EUR 262 |
 Taq Plus DNA Polymerase, 5u/ul |
|
D0090 |
Bio Basic |
200U |
EUR 79 |
|
|
 Maximo DFS-Plus Taq DNA Polymerase DNA-free |
|
N140 |
GeneOn |
500 units |
EUR 50 |
Maximo DFS-Plus Taq DNA Polymerase DNA-free |
|
N142 |
GeneOn |
5x500 units |
EUR 120 |
Maximo DFS-Plus Taq DNA Polymerase DNA-free |
|
N144 |
GeneOn |
20x500 units |
EUR 367 |
 Taq DNA Polymerase |
|
9001-2500 |
Biovision |
|
EUR 512 |
Taq DNA Polymerase |
|
9001-500 |
Biovision |
|
EUR 191 |
Taq DNA Polymerase |
|
L7051001 |
Biochain |
1000 unit |
EUR 195 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Taq DNA Polymerase |
|
L7051200 |
Biochain |
200 unit |
EUR 87 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Taq DNA Polymerase |
|
BA00103 |
Cusabio |
500U |
EUR 87 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA Polymerase |
|
BA00104 |
Bioatlas |
1000U |
EUR 123 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA Polymerase |
|
BA00105 |
Bioatlas |
2500U |
EUR 232 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
 Taq DNA polymerase |
|
BT10101 |
Bioatlas |
500U |
EUR 80 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA polymerase |
|
BT10102 |
Bioatlas |
1000U |
EUR 109 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
Taq DNA polymerase |
|
BT10103 |
Bioatlas |
2000U |
EUR 167 |
|
Description: High quality Taq polymerase for different PCR variations and downstream applications. |
) Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP) |
|
P101-d1 |
Vazyme |
1000 U |
EUR 136 |
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP) |
|
P101-d2 |
Vazyme |
5000 U |
EUR 267 |
Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP) |
|
P101-d3 |
Vazyme |
10000 U |
EUR 409 |
 Champagne Taq DNA Polymerase |
|
P122-d1 |
Vazyme |
500 U (2.5 U/μl) |
EUR 177 |
Champagne Taq DNA Polymerase |
|
P122-d2 |
Vazyme |
500 U (5 U/μl) |
EUR 177 |
Champagne Taq DNA Polymerase |
|
P122-d3 |
Vazyme |
500 U (10 U/μl) |
EUR 177 |
 Hotstart Taq DNA Polymerase |
|
L7052001 |
Biochain |
1000 units |
EUR 568 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
Hotstart Taq DNA Polymerase |
|
L7052250 |
Biochain |
250 units |
EUR 195 |
|
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol. |
) Taq DNA Polymerase (500U) |
|
9K-001-0001 |
Bio Basic |
500U |
EUR 174.41 |
|
|
) Taq DNA Polymerase (3000U) |
|
9K-001-0002 |
Bio Basic |
3000U |
EUR 485 |
|
|
) Taq DNA Polymerase (6000U) |
|
9K-001-0033 |
Bio Basic |
6000U |
EUR 1028.75 |
|
|
) Taq DNA Polymerase (1000U) |
|
9K-001-0035 |
Bio Basic |
1000U |
EUR 348.85 |
|
|
) Taq DNA Polymerase (Recombinant) |
|
abx073521-10000U |
Abbexa |
10000 U |
EUR 592 |
|
|
Taq DNA Polymerase (Recombinant) |
|
abx073521-1000U |
Abbexa |
1000 U |
EUR 230 |
|
|
Taq DNA Polymerase (Recombinant) |
|
abx073521-3000U |
Abbexa |
3000 U |
EUR 328 |
|
|
 Recombinant Taq DNA Polymerase |
|
7-03634 |
CHI Scientific |
1,000U |
Ask for price |
Recombinant Taq DNA Polymerase |
|
7-03635 |
CHI Scientific |
3,000U |
Ask for price |
Recombinant Taq DNA Polymerase |
|
7-03636 |
CHI Scientific |
10,000U |
Ask for price |
) Taq DNA polymerase (+dNTPs) |
|
02-001 |
Sceti |
200U |
EUR 153 |
|
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur. |
Taq DNA polymerase (+dNTPs) |
|
02-001-5 |
Sceti |
5×200U |
EUR 257 |
|
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur. |
) Taq DNA polymerase (-dNTPs) |
|
02-011 |
Sceti |
200U |
EUR 140 |
|
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur. |
Taq DNA polymerase (-dNTPs) |
|
02-011-5 |
Sceti |
5×200U |
EUR 218 |
|
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur. |
 Maximo Taq DNA Polymerase |
|
S101 |
GeneOn |
500 units |
EUR 46 |
Maximo Taq DNA Polymerase |
|
S102 |
GeneOn |
5x500 units |
EUR 107 |
Maximo Taq DNA Polymerase |
|
S103 |
GeneOn |
20x500 units |
EUR 328 |
 Taq DNA Polymerase, 5units/㎕ |
|
T2200-050 |
GenDepot |
500units |
EUR 131 |
Taq DNA Polymerase, 5units/㎕ |
|
T2200-100 |
GenDepot |
2x500 units |
EUR 192 |
Taq DNA Polymerase, 5units/㎕ |
|
T2200-200 |
GenDepot |
4x500 units |
EUR 313 |
 2X PCR Taq Plus MasterMix |
|
G014 |
ABM |
5.0 ml (200 Rxns) |
EUR 106 |
2X PCR Taq Plus MasterMix |
|
G901 |
ABM |
25.0 ml (1000 Rxns) |
EUR 299 |
) Taq Plus DNA Polmerase (with dNTP) |
|
P201-d1 |
Vazyme |
250 U |
EUR 126 |
Taq Plus DNA Polmerase (with dNTP) |
|
P201-d2 |
Vazyme |
1000 U |
EUR 193 |
Taq Plus DNA Polmerase (with dNTP) |
|
P201-d3 |
Vazyme |
3000 U |
EUR 354 |
 Taq DNA Polymerase Polyclonal Antibody |
|
7018-100 |
Biovision |
|
EUR 354 |
Taq DNA Polymerase Polyclonal Antibody |
|
7018-30T |
Biovision |
|
EUR 146 |
) Fast-Taq DNA Polymerase (500U) |
|
9K-001-0036 |
Bio Basic |
500U |
EUR 350.54 |
|
|
 Taq DNA Polymerase, 5u/ul |
|
B0089(D0089) |
Bio Basic |
5X200U |
EUR 93.5 |
|
|
 ACTaq? Taq DNA Polymerase, 1000U |
|
E2100-1000U |
ACTGene |
|
EUR 217 |
 ACTaq? Taq DNA Polymerase, 250U |
|
E2100-250U |
ACTGene |
|
EUR 102 |
 Anti-Taq DNA Polymerase Antibody |
|
48369-100ul |
SAB |
100ul |
EUR 333 |
Anti-Taq DNA Polymerase Antibody |
|
48369-50ul |
SAB |
50ul |
EUR 239 |
 KD Plus DNA Polymerase Enzyme |
|
abx071022-100U |
Abbexa |
100 U |
EUR 244 |
|
|
KD Plus DNA Polymerase Enzyme |
|
abx071022-1200U |
Abbexa |
1200 U |
EUR 899 |
|
|
KD Plus DNA Polymerase Enzyme |
|
abx071022-200U |
Abbexa |
200 U |
EUR 314 |
|
|
 , DNA Aptamer, Biotinylated) Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated |
|
AD-146-B |
Alpha Diagnostics |
Custom |
Ask for price |
 , DNA Aptamer, unlabeled) Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled |
|
AD-146-U |
Alpha Diagnostics |
Custom |
Ask for price |
 Accuris Taq Plus |
|
PR1000-TP-250 |
BenchMark |
1 PC |
EUR 194.99 |
|
|
Accuris Taq Plus |
|
PR1000-TP-500 |
BenchMark |
1 PC |
EUR 273.15 |
|
|
Accuris Taq Plus |
|
PR1000-TP-S |
BenchMark |
1 PC |
EUR 69.79 |
|
|
) Taq DNA Polymerase (Mg2+ free Buffer) |
|
P102-01 |
Vazyme |
1000 U |
EUR 121 |
Taq DNA Polymerase (Mg2+ free Buffer) |
|
P102-02 |
Vazyme |
5000 U |
EUR 193 |
Taq DNA Polymerase (Mg2+ free Buffer) |
|
P102-03 |
Vazyme |
10000 U |
EUR 262 |
 Cheetah Hot Start Taq DNA Polymerase |
|
29050 |
Biotium |
500EU |
EUR 296 |
|
Description: Minimum order quantity: 1 unit of 500EU |
 amfiSure Hot-Start Taq DNA Polymerase |
|
P0322-010 |
GenDepot |
100 units |
EUR 147 |
amfiSure Hot-Start Taq DNA Polymerase |
|
P0322-025 |
GenDepot |
250 units |
EUR 228 |
 ACTaq? Taq Blue DNA Polymerase, 1000U |
|
E2100B-1000U |
ACTGene |
|
EUR 227 |
 ACTaq? Taq Blue DNA Polymerase, 250U |
|
E2100B-250U |
ACTGene |
|
EUR 105 |
 Taq DNA Polymerase, 5units/㎕ , Bulk Packing |
|
T2200-B01 |
GenDepot |
10,000 units |
EUR 1157 |
Taq DNA Polymerase, 5units/㎕ , Bulk Packing |
|
T2200-B05 |
GenDepot |
5x10,000 units |
EUR 5450 |
 HiQTM Taq Red DNA Polymerase, 1unit/㎕ |
|
T2201-050 |
GenDepot |
500units |
EUR 131 |
HiQTM Taq Red DNA Polymerase, 1unit/㎕ |
|
T2201-100 |
GenDepot |
2x500 units |
EUR 192 |
HiQTM Taq Red DNA Polymerase, 1unit/㎕ |
|
T2201-200 |
GenDepot |
4x500 units |
EUR 313 |
 Taq DNA Polymerase, 5 units/µl |
|
ABP-PP-ERTQ001 |
Allele Biotech |
1000U |
Ask for price |
|
|
Taq DNA Polymerase, 5 units/µl |
|
ABP-PP-ERTQ100 |
Allele Biotech |
100U |
Ask for price |
|
|
Taq DNA Polymerase, 5 units/µl |
|
ABP-PP-ERTQ200 |
Allele Biotech |
200U |
Ask for price |
|
|
Taq DNA Polymerase, 5 units/µl |
|
ABP-PP-ERTQ500 |
Allele Biotech |
500U |
Ask for price |
|
|
 Taq Plus DNA Polymerase)
 Taq DNA Polymerase)
