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August 26, 2020

Treatment of Polymers Enables Reagent-Free Covalent

By Brandy

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WordPress database error: [Unknown column 'main_image' in 'field list']
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Atmospheric Pressure Plasma Jet Treatment of Polymers Enables Reagent-Free Covalent Attachment of Biomolecules for Bioprinting

3D bioprinting, where cells, hydrogels and structural polymers can be printed layer by layer into complex designs, holds great promise for advances in medicine and the biomedical sciences. In principle, this technique enables the creation of highly patient specific disease models and biomedical implants. However, an ability to tailor surface biocompatibility and interfacial bonding between printed components, such as polymers and hydrogels, is currently lacking.
  • Here we demonstrate that an atmospheric pressure plasma jet (APPJ) can locally activate polymeric surfaces for the reagent-free covalent attachment of proteins and hydrogel in a single-step process at desired locations. Polyethylene and poly--caprolactone (PCL) were used as example polymers. Covalent attachment of the proteins and hydrogel was demonstrated by resistance to removal by rigorous sodium dodecyl sulfate (SDS) washing.
  • The immobilized protein and hydrogel layers were analyzed using Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS). Importantly, the APPJ surface activation also rendered the polymer surfaces mildly hydrophilic as required for optimum biocompatibility.
  • Water contact angles were observed to be stable within a range where the conformation of biomolecules is preserved. Single and double electrode designs of APPJs were compared in their characteristics relevant to localized surface functionalization, plume length and shape. As a proof of efficacy in a biological context, APPJ treated polyethylene functionalized with fibronectin was used to demonstrate improvements in cell adhesion and proliferation.
  • These results have important implications for the development of a new generation of 3D bioprinters capable of spatially patterned and tailored surface functionalization performed during the 3D printing process in situ.
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Advanced glycosylation end product-specific receptor Polyclonal Antibody
42577-100ul 100ul
EUR 399.6

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx171123
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  • EUR 493.20
  • 1 mg
  • 200 ug

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx104577
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  • EUR 1328.40
  • EUR 644.40
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  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx104578
  • EUR 493.20
  • EUR 159.60
  • EUR 1362.00
  • EUR 661.20
  • EUR 376.80
  • 100 ug
  • 10 ug
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  • 50 ug

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx001218
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  • 100 ul
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Advanced Glycosylation End Product-Specific Receptor (AGER) Antibody
20-abx109506
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  • EUR 2214.00
  • EUR 718.80
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  • 100 ug
  • 1 mg
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  • 50 ug

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx130064
  • EUR 360.00
  • EUR 159.60
  • EUR 961.20
  • EUR 510.00
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  • 100 ug
  • 10 ug
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  • 200 ug
  • 50 ug

Advanced Glycosylation End Product-Specific Receptor (AGER) Antibody
abx031586-400ul 400 ul
EUR 627.6

Advanced Glycosylation End Product-Specific Receptor (AGER) Antibody
abx031586-80l 80 µl
EUR 343.2

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
abx032323-400ul 400 ul
EUR 627.6

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
abx032323-80l 80 µl
EUR 343.2

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
abx036573-100ug 100 ug
EUR 469.2

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
abx230209-100ug 100 ug
EUR 577.2

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx324550
  • EUR 376.80
  • EUR 292.80
  • 100 ug
  • 50 ug

Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx211205
  • EUR 493.20
  • EUR 360.00
  • 100 ul
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Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx211206
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  • 100 ul
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Advanced Glycosylation End Product Specific Receptor (AGER) Antibody
20-abx175289
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  • 1 mg
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Advanced Glycation End Product (AGE) Polyclonal Antibody (General)
4-PAB353Ge01
  • EUR 283.20
  • EUR 2805.60
  • EUR 703.20
  • EUR 352.80
  • EUR 250.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE)

Advanced Glycation End Product (AGE) Monoclonal Antibody (General)
4-MAB353Ge21
  • EUR 292.80
  • EUR 2964.00
  • EUR 739.20
  • EUR 367.20
  • EUR 254.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE)

Advanced Glycation End Product (AGE) ELISA Kit
20-abx150316
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Advanced Glycation End Product (AGE) ELISA Kit
abx255064-96tests 96 tests
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Advanced Glycosylation End Product-Specific Receptor Protein
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Advanced Glycation End Product (AGE) ELISA Kit
abx576530-96tests 96 tests
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Advanced Glycation End Product (AGE) Protein (Active)
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  • 100 ug
  • 10 ug
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  • 50 ug

Advanced Glycation End Product (AGE) ELISA Kit
DLR-AGE-Ge-48T 48T
EUR 627.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma, tissue homogenates or other biological fluids.

Advanced Glycation End Product (AGE) ELISA Kit
DLR-AGE-Ge-96T 96T
EUR 817.2
Description: A competitive inhibition quantitative ELISA assay kit for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma, tissue homogenates or other biological fluids.

Pig Advanced Glycosylation End Products ELISA kit
E07A0002-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Advanced Glycosylation End Products ELISA kit
E07A0002-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Advanced Glycosylation End Products ELISA kit
E07A0002-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Advanced Glycosylation End Products ELISA kit
E06A0002-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Advanced Glycosylation End Products ELISA kit
E09A0002-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Advanced Glycosylation End Products ELISA kit
E09A0002-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Advanced Glycosylation End Products ELISA kit
E09A0002-96 1 plate of 96 wells
EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Advanced Glycosylation End Products ELISA kit
E08A0002-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Advanced Glycosylation End Products ELISA kit
E08A0002-48 1 plate of 48 wells
EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Advanced Glycosylation End Products ELISA kit
E08A0002-96 1 plate of 96 wells
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Description: A sandwich ELISA for quantitative measurement of Canine Advanced Glycosylation End Products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Advanced Glycation End Products (AGEs) Assay Kit
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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC.

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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Biotin.

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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Cy3.

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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with FITC.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), HRP
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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with HRP.

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  • EUR 423.60
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  • 100ul
  • 10ml
  • 1ml
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Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with PE.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), APC
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  • 100ul
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), Biotinylated
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  • EUR 860.40
  • EUR 452.40
  • EUR 260.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Biotin.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), Cy3
4-MAB353Ge21-Cy3
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  • EUR 5103.60
  • EUR 1388.40
  • EUR 644.40
  • EUR 297.60
  • 100ul
  • 10ml
  • 1ml
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Cy3.

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  • EUR 351.60
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  • EUR 886.80
  • EUR 440.40
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
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Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with FITC.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), HRP
4-MAB353Ge21-HRP
  • EUR 374.40
  • EUR 3369.60
  • EUR 954.00
  • EUR 471.60
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with HRP.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), PE
4-MAB353Ge21-PE
  • EUR 351.60
  • EUR 3115.20
  • EUR 886.80
  • EUR 440.40
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with PE.

Recombinant Human Advanced Glycosylation End Product-Specific Receptor
7-06520 2µg Ask for price

Recombinant Human Advanced Glycosylation End Product-Specific Receptor
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Human Advanced Glycation End Product (AGE) ELISA Kit
20-abx054077
  • EUR 8853.60
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  • 10 × 96 tests
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  • 96 tests

Advanced Glycosylation End Product-Specific Receptor, HEK Protein
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  • EUR 1646.40
  • EUR 393.60
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  • 100 ug
  • 10 ug
  • 2 µg

Human Advanced Glycation End Product (AGE) ELISA Kit
abx253588-96tests 96 tests
EUR 801.6

Human Advanced Glycation End Product (AGE) ELISA Kit
abx512405-96tests 96 tests
EUR 801.6

Mouse Advanced Glycation End Product (AGE) ELISA Kit
abx512406-96tests 96 tests
EUR 782.4

Rat Advanced Glycation End Product (AGE) ELISA Kit
abx512407-96tests 96 tests
EUR 782.4

Rat Advanced Glycation End Product (AGE) ELISA Kit
abx256246-96tests 96 tests
EUR 904.8

Human advanced glycation end products,AGEs ELISA Kit
201-12-0004 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Rat advanced glycation end products,AGEs ELISA Kit
CN-01677R1 96T
EUR 549.6

Rat advanced glycation end products,AGEs ELISA Kit
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EUR 368.4

Human advanced glycation end products, AGEs ELISA Kit
CSB-E09412h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human advanced glycation end products, AGEs ELISA Kit
1-CSB-E09412h
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat advanced glycation end products, AGEs ELISA Kit
CSB-E09413r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat advanced glycation end products, AGEs ELISA Kit
1-CSB-E09413r
  • EUR 1160.40
  • EUR 7110.00
  • EUR 3760.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse advanced glycation end products, AGEs ELISA Kit
CSB-E09414m-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse advanced glycation end products, AGEs ELISA Kit
1-CSB-E09414m
  • EUR 1135.20
  • EUR 6938.40
  • EUR 3672.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

General Advanced Glycation End Product (AGE) ELISA Kit
CEB353Ge-10x96wellstestplate 10x96-wells test plate
EUR 5764.2
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
CEB353Ge-1x48wellstestplate 1x48-wells test plate
EUR 579.88
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
CEB353Ge-1x96wellstestplate 1x96-wells test plate
EUR 776.96
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
CEB353Ge-5x96wellstestplate 5x96-wells test plate
EUR 3136.34
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
4-CEB353Ge
  • EUR 5824.80
  • EUR 3076.80
  • EUR 777.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General Advanced Glycation End Product (AGE) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Human advanced glycation end products,AGEs ELISA Kit
CN-04128H1 96T
EUR 567.6

Human advanced glycation end products,AGEs ELISA Kit
CN-04128H2 48T
EUR 387.6

Mouse advanced glycation end products,AGEs ELISA Kit
CN-02549M1 96T
EUR 565.2

Mouse advanced glycation end products,AGEs ELISA Kit
CN-02549M2 48T
EUR 386.4

Human Advanced glycosylation end product-specific receptor (AGER)
1-CSB-RP142074h
  • EUR 456.00
  • EUR 256.80
  • EUR 1570.80
  • EUR 672.00
  • EUR 1047.60
  • EUR 314.40
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
Description: Recombinant Human Advanced glycosylation end product-specific receptor(AGER),partial expressed in E.coli

Goat Hemoglobin advanced glycosylation end products ELISA kit
E06H0219-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Hemoglobin advanced glycosylation end products ELISA kit
E06H0219-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Hemoglobin advanced glycosylation end products ELISA kit
E06H0219-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Hemoglobin advanced glycosylation end products ELISA kit
E04H0219-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Hemoglobin advanced glycosylation end products ELISA kit
E04H0219-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Hemoglobin advanced glycosylation end products ELISA kit
E04H0219-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Hemoglobin advanced glycosylation end products ELISA kit
E01H0219-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Hemoglobin advanced glycosylation end products ELISA kit
E01H0219-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Hemoglobin advanced glycosylation end products ELISA kit
E01H0219-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Hemoglobin advanced glycosylation end products in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat AGE/ Advanced glycation end-product ELISA Kit
E0037Ra 1 Kit
EUR 685.2

Improved HPLC Conditions to Determine Eumelanin and Pheomelanin Contents in Biological Samples Using an Ion Pair Reagent

Alkaline hydrogen peroxide oxidation (AHPO) of eumelanin and pheomelanin, two major classes of melanin pigments, affords pyrrole-2,3,5-tricarboxylic acid (PTCA), pyrrole-2,3-dicarboxylic acid (PDCA) and pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA) from eumelanin and thiazole-2,4,5-tricarboxylic acid (TTCA) and thiazole-4,5-dicarboxylic acid (TDCA) from pheomelanin.
Quantification of these five markers by HPLC provides useful information on the quantity and structural diversity of melanins in various biological samples. HPLC analysis of these markers using the original method of 0.1 M potassium phosphate buffer (pH 2.1):methanol = 99:1 (85:15 for PTeCA) on a reversed-phase column had some problems, including the short lifetime of the column and, except for the major eumelanin marker PTCA, other markers were occasionally overlapped by interfering peaks in samples containing only trace levels of these markers.
These problems can be overcome by the addition of an ion pair reagent for anions, such as tetra-n-butylammonium bromide (1 mM), to retard the elution of di-, tri- and tetra-carboxylic acids. The methanol concentration was increased to 17% (30% for PTeCA) and the linearity, reproducibility, and recovery of the markers with this improved method is good to excellent.
 This improved HPLC method was compared to the original method using synthetic melanins, mouse hair, human hair, and human epidermal samples. In addition to PTCA, TTCA, a major marker for pheomelanin, showed excellent correlations between both HPLC methods. The other markers showed an attenuation of the interfering peaks with the improved method. We recommend this improved HPLC method for the quantitative analysis of melanin markers following AHPO because of its simplicity, accuracy, and reproducibility.
Keywords: 4-amino-3-hydroxyphenylalanine (4-AHP); alkaline hydrogen peroxide oxidation (AHPO); high-performance liquid chromatography (HPLC); individual typology angle (ITA); pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA); pyrrole-2,3,5-tricarboxylic acid (PTCA); pyrrole-2,3-dicarboxylic acid (PDCA); thiazole-2,4,5-tricarboxylic acid (TTCA); thiazole-4,5-dicarboxylic acid (TDCA); total melanin (TM).

Manipulating the click reactivity of dibenzoazacyclooctynes: from an azide clicker to a caged acylation reagent

Strain-promoted azide-alkyne cycloaddition using dibenzoazacyclooctyne (DBCO) is widely applied in copper-free bioorthogonal reactions. Here, we report the efficient acid-promoted rearrangement and silver-catalyzed amidation of DBCO, which alters its click reactivity robustly. In the switched click reaction, DBCO, as a caged acylation reagent, enables rapid peptide/protein modification after decaged by silver catalysts that renders site-specific conjugation of IgG antibody via an Fc-targeting peptide.
Keywords: DBCO; caged acylation reagent; click chemistry; silver catalyst; switched click reaction.

Myoglobin-Free Serum Protein
abx069893-50ml 50 ml
EUR 1345.2

VitroPlus III, Serum-Free, Xeno-Free, Complete
PC00B2 500 ml
EUR 450

Human Free PSA (f-PSA) ELISA Kit
PRB-5049-FREE-5 5 x 96 assays
EUR 2739.6

Human Serum (Troponin I Free)
90R-106 100 ml
EUR 1417.2
Description: Troponion I free normal human serum

Serum Albumin, Lipid Free Protein
20-abx260049
  • EUR 927.60
  • EUR 343.20
  • EUR 627.60
  • 100 mg
  • 10 mg
  • 50 mg

Bovine Serum Albumin, protease free
GK4012-1KG 1 kg
EUR 1165.2

Bovine Serum Albumin, protease free
GK4012-500G 500 g
EUR 638.4

PK15 Cell Serum-Free Medium
VCum-Lsx0007 1 L
EUR 909.6
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

CHO Cell Serum-Free Medium
VCum-Lsx0025 1L
EUR 762
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium
VCum-Lsx0027 1L
EUR 762
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium
VCum-Lsx0029 1L
EUR 818.4
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium
VCum-Lsx0031 1 L
EUR 909.6
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

500mL Insectagro DS2 Serum-Free
13-402-CV PK6
EUR 222.3

500mL INSECTAGRO SF9 serum free
13-410-CV PK6
EUR 192.66

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-1 1 ml
EUR 343.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-10 10 ml
EUR 781.2

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-5 5 ml
EUR 634.8

EZ-DNA Reagents
BS8202 100preps
EUR 105.67

EZ-DNA Reagents
SK8201 100preps
EUR 112.2

Ecl Detection Reagents
RPN2105 EACH
EUR 611.04

Ecl Detection Reagents
RPN3004 EACH
EUR 489.06

Rabbit Serum
abx098265-100ml 100 ml
EUR 326.4

Rabbit Serum
abx098266-500ml 500 ml
EUR 577.2

Rabbit Serum
1267-1 each
EUR 188.4

B-27 Serum-Free Supplement (50x)
abx082466-10ml 10 ml
EUR 393.6

Normal Bovine Serum (Protease/IgG free)
88R-1012 10 ml
EUR 218.4
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Fetal Bovine Serum, Premium Tetracyclin Free
F0500-050 500ml
EUR 1388.4

Bovine Serum Albumin, fatty acid free
GX5685-1KG 1 kg
EUR 1442.4

Bovine Serum Albumin, fatty acid free
GX5685-500G 500 g
EUR 792

ST Cell Serum-Free Medium-1
VCum-Lsx0001 1 L
EUR 888
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2
VCum-Lsx0003 1 L
EUR 909.6
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-10L 10 L
EUR 2798.4
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-1L 1 L
EUR 333.6
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

PK15 Cell Serum-Free Medium (Powder)
VCum-Lsx0008-5L 5 L
EUR 1429.2
Description: PK15 cell serum-free medium has been specifically developed for suspension culture of PK-15 cells.

MDCK Cell Serum-Free Medium-1
VCum-Lsx0009 1 L
EUR 818.4
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-2
VCum-Lsx0011 1 L
EUR 832.8
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

BHK Cell Serum-Free Medium-1
VCum-Lsx0017 1 L
EUR 776.4
Description: BHK cell serum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-2
VCum-Lsx0019 1 L
EUR 860.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

Insect Cell Serum-Free Medium-1
VCum-Lsx0021 1 L
EUR 888
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2
VCum-Lsx0023 1 L
EUR 909.6
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-10L 10 L
EUR 2096.4
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-1L 1 L
EUR 264
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

CHO Cell Serum-Free Medium (Powder)
VCum-Lsx0026-5L 5 L
EUR 1077.6
Description: A serum-free medium that is ideal for suspension culture of CHO cell lines (CHO DG44, CHO-K1 and CHO-S).

HEK Cell Serum-Free Medium (Powder)
VCum-Lsx0028 50 L
EUR 7693.2
Description: A serum-free medium that is ideal for suspension culture of HEK cell lines.

MDBK Cell Serum-Free Medium (Powder)
VCum-Lsx0030 50 L
EUR 10309.2
Description: Used in serum-free suspension MDBK cell line culture for bovine virus diarrhea or bovine rhinotracheitis vaccine production.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-10L 10 L
EUR 2798.4
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-1L 1 L
EUR 338.4
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Vero Cell Serum-Free Medium (Powder)
VCum-Lsx0032-5L 5 L
EUR 1449.6
Description: A serum-free medium that is ideal for suspension culture of Vero cell lines.

Corning Lymphocyte Serum Free Medium KBM551
88-551-CM 1L
EUR 182.4

Corning Lymphocyte Serum Free Medium KBM581
88-581-CM 1L
EUR 240.54

REAGENTS HIGH RANGE AMMONIA
HI73325 PK25
EUR 38.19

High Range Ammonia Reagents
HI9373301 EACH
EUR 91.2

Alkphos Direct Labelling Reagents
RPN3680 EACH
EUR 686.28

Rabbit(Free-T3)
QY-E30315 96T
EUR 448.8

Human Apolipoprotein AI / B Free Serum Protein
abx060869-100ml 100 ml
EUR 594

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-10L 10 L
EUR 2658
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-1L 1 L
EUR 319.2
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0002-5L 5 L
EUR 1358.4
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-10L 10 L
EUR 2798.4
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-1L 1 L
EUR 333.6
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

ST Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0004-5L 5 L
EUR 1429.2
Description: ST cell serum-free medium has been specifically developed for suspension culture of ST cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-10L 10 L
EUR 2236.8
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-1L 1 L
EUR 277.2
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0010-5L 5 L
EUR 1148.4
Description: MDCK cell serum-free medium has been specifically developed for suspension culture of MDCK cells.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-10L 10 L
EUR 2306.4
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-1L 1 L
EUR 284.4
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

MDCK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0012-5L 5 L
EUR 1183.2
Description: Used in serum-free suspension MDCK cell line culture for avian influenza vaccine production.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-10L 10 L
EUR 2025.6
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-1L 1 L
EUR 256.8
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0018-5L 5 L
EUR 1042.8
Description: BHK cellserum-free medium has been specifically developed for suspension culture of BHK cells.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-10L 10 L
EUR 2306.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-1L 1 L
EUR 284.4
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

BHK Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0020-5L 5 L
EUR 1183.2
Description: Used in serum-free suspension BHK cell line culture for newcastle disease virus production.

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-10L 10 L
EUR 2658
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-1L 1 L
EUR 319.2
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-1 (Powder)
VCum-Lsx0022-5L 5 L
EUR 1358.4
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-10L 10 L
EUR 2798.4
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-1L 1 L
EUR 333.6
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Insect Cell Serum-Free Medium-2 (Powder)
VCum-Lsx0024-5L 5 L
EUR 1429.2
Description: A serum-free medium that is ideal for suspension culture of insect cell lines (HighFive, SF9).

Rabbit Complement Serum
32R-CR001 25 ml
EUR 289.2
Description: Purifed Rabbit Complement isolated from blood of New Zealand white rabbits

Rabbit Serum Albumin
30R-3303 10 mg
EUR 224.4
Description: Purified native Rabbit Serum Albumin

Rabbit Serum (Sterile)
20-abx800002
  • EUR 1680.00
  • EUR 3951.60
  • EUR 994.80
  • 100 ml
  • 500 ml
  • 50 ml

Rabbit Serum (Sterile)
abx800002-1L 1 L
EUR 6524.4

Normal Rabbit Serum
88-NR50 100 ml
EUR 180
Description: Normal Rabbit Serum

Normal Rabbit Serum
88R-1004 10 ml
EUR 247.2
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
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