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August 26, 2020

Treatment of Polymers Enables Reagent-Free Covalent

By Brandy

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Atmospheric Pressure Plasma Jet Treatment of Polymers Enables Reagent-Free Covalent Attachment of Biomolecules for Bioprinting

3D bioprinting, where cells, hydrogels and structural polymers can be printed layer by layer into complex designs, holds great promise for advances in medicine and the biomedical sciences. In principle, this technique enables the creation of highly patient specific disease models and biomedical implants. However, an ability to tailor surface biocompatibility and interfacial bonding between printed components, such as polymers and hydrogels, is currently lacking.
  • Here we demonstrate that an atmospheric pressure plasma jet (APPJ) can locally activate polymeric surfaces for the reagent-free covalent attachment of proteins and hydrogel in a single-step process at desired locations. Polyethylene and poly--caprolactone (PCL) were used as example polymers. Covalent attachment of the proteins and hydrogel was demonstrated by resistance to removal by rigorous sodium dodecyl sulfate (SDS) washing.
  • The immobilized protein and hydrogel layers were analyzed using Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS). Importantly, the APPJ surface activation also rendered the polymer surfaces mildly hydrophilic as required for optimum biocompatibility.
  • Water contact angles were observed to be stable within a range where the conformation of biomolecules is preserved. Single and double electrode designs of APPJs were compared in their characteristics relevant to localized surface functionalization, plume length and shape. As a proof of efficacy in a biological context, APPJ treated polyethylene functionalized with fibronectin was used to demonstrate improvements in cell adhesion and proliferation.
  • These results have important implications for the development of a new generation of 3D bioprinters capable of spatially patterned and tailored surface functionalization performed during the 3D printing process in situ.
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Native Advanced Glycation End Product (AGE)
4-NPB353Ge01
  • EUR 442.56
  • EUR 242.40
  • EUR 1329.60
  • EUR 523.20
  • EUR 926.40
  • EUR 372.00
  • EUR 3144.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
Description: Recombinant Pan-species Advanced Glycation End Product expressed in: Natural extract

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), PE
4-MAB353Ge21-PE
  • EUR 351.60
  • EUR 3115.20
  • EUR 886.80
  • EUR 440.40
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with PE.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), PE
4-PAB353Ge01-PE
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with PE.

Advanced Glycation End Product (AGE) Protein
20-abx168580
  • EUR 627.60
  • EUR 292.80
  • EUR 1796.40
  • EUR 727.20
  • EUR 460.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), APC
4-MAB353Ge21-APC
  • EUR 409.20
  • EUR 3865.20
  • EUR 1078.80
  • EUR 520.80
  • EUR 260.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), Cy3
4-MAB353Ge21-Cy3
  • EUR 496.80
  • EUR 5103.60
  • EUR 1388.40
  • EUR 644.40
  • EUR 297.60
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Cy3.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), FITC
4-MAB353Ge21-FITC
  • EUR 351.60
  • EUR 3115.20
  • EUR 886.80
  • EUR 440.40
  • EUR 232.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with FITC.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), HRP
4-MAB353Ge21-HRP
  • EUR 374.40
  • EUR 3369.60
  • EUR 954.00
  • EUR 471.60
  • EUR 246.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with HRP.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), APC
4-PAB353Ge01-APC
  • EUR 394.80
  • EUR 3649.20
  • EUR 1024.80
  • EUR 499.20
  • EUR 254.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), Cy3
4-PAB353Ge01-Cy3
  • EUR 476.40
  • EUR 4815.60
  • EUR 1316.40
  • EUR 615.60
  • EUR 289.20
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Cy3.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), FITC
4-PAB353Ge01-FITC
  • EUR 339.60
  • EUR 2942.40
  • EUR 843.60
  • EUR 423.60
  • EUR 226.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with FITC.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), HRP
4-PAB353Ge01-HRP
  • EUR 362.40
  • EUR 3182.40
  • EUR 907.20
  • EUR 452.40
  • EUR 240.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with HRP.

Advanced Glycation End Product (AGE) CLIA Kit
20-abx490411
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Advanced Glycation End Product (AGE) ELISA Kit
abx255064-96tests 96 tests
EUR 895.2

Advanced Glycation End Product (AGE) ELISA Kit
20-abx150316
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Advanced Glycation End Product (AGE) ELISA Kit
abx576530-96tests 96 tests
EUR 895.2

Advanced Glycation End Product (AGE) ELISA Kit
DLR-AGE-Ge-48T 48T
EUR 627.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma, tissue homogenates or other biological fluids.

Advanced Glycation End Product (AGE) ELISA Kit
DLR-AGE-Ge-96T 96T
EUR 817.2
Description: A competitive inhibition quantitative ELISA assay kit for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma, tissue homogenates or other biological fluids.

Advanced Glycation End Products (AGEs) Assay Kit
K929-100 each
EUR 502.8

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), APC-Cy7
4-MAB353Ge21-APC-Cy7
  • EUR 675.60
  • EUR 7586.40
  • EUR 2013.60
  • EUR 898.80
  • EUR 378.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC-Cy7.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), APC-Cy7
4-PAB353Ge01-APC-Cy7
  • EUR 645.60
  • EUR 7154.40
  • EUR 1905.60
  • EUR 855.60
  • EUR 364.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC-Cy7.

Advanced Glycation End Product (AGE) Monoclonal Antibody (General), Biotinylated
4-MAB353Ge21-Biotin
  • EUR 370.80
  • EUR 2904.00
  • EUR 860.40
  • EUR 452.40
  • EUR 260.40
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Biotin.

Advanced Glycation End Product (AGE) Polyclonal Antibody (General), Biotinylated
4-PAB353Ge01-Biotin
  • EUR 358.80
  • EUR 2745.60
  • EUR 820.80
  • EUR 435.60
  • EUR 256.80
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Biotin.

Advanced Glycation End Product (AGE) Protein (Active)
20-abx651294
  • EUR 777.60
  • EUR 326.40
  • EUR 2331.60
  • EUR 910.80
  • EUR 560.40
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Advanced Glycation End Product (AGE) ELISA Kit
abx256246-96tests 96 tests
EUR 904.8

Rat Advanced Glycation End Product (AGE) ELISA Kit
abx512407-96tests 96 tests
EUR 782.4

for Advanced Glycation End Product (AGE)ELISA kit
HEB353Ge-10x96wellstestplate 10x96-wells test plate
EUR 6321.83
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

for Advanced Glycation End Product (AGE)ELISA kit
HEB353Ge-1x48wellstestplate 1x48-wells test plate
EUR 625.8
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

for Advanced Glycation End Product (AGE)ELISA kit
HEB353Ge-1x96wellstestplate 1x96-wells test plate
EUR 842.57
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

for Advanced Glycation End Product (AGE)ELISA kit
HEB353Ge-5x96wellstestplate 5x96-wells test plate
EUR 3431.56
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of for Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

ELISA Kit for Advanced Glycation End Product (AGE)
4-HEB353Ge
  • EUR 6382.80
  • EUR 3372.00
  • EUR 843.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Cat Advanced Glycation End Product (AGE) ELISA Kit
YLA0032CT-48T 48T
EUR 547.5

Cat Advanced Glycation End Product (AGE) ELISA Kit
YLA0032CT-96T 96T
EUR 637.5

Human Advanced Glycation End Product (AGE) ELISA Kit
abx253588-96tests 96 tests
EUR 801.6

Human Advanced Glycation End Product (AGE) ELISA Kit
20-abx054077
  • EUR 8853.60
  • EUR 4719.60
  • EUR 1093.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Advanced Glycation End Product (AGE) ELISA Kit
abx512405-96tests 96 tests
EUR 801.6

Mouse Advanced Glycation End Product (AGE) ELISA Kit
abx512406-96tests 96 tests
EUR 782.4

Rat advanced glycation end products,AGEs ELISA Kit
CN-01677R1 96T
EUR 549.6

Rat advanced glycation end products,AGEs ELISA Kit
CN-01677R2 48T
EUR 368.4

Rat advanced glycation end products, AGEs ELISA Kit
CSB-E09413r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat advanced glycation end products, AGEs ELISA Kit
1-CSB-E09413r
  • EUR 1160.40
  • EUR 7110.00
  • EUR 3760.80
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat AGE(Advanced glycation end-product) ELISA Kit
ER0268 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 0.188 ng/ml

Rat AGE/ Advanced glycation end-product ELISA Kit
E0037Ra 1 Kit
EUR 685.2

Rat advanced glycation end products(AGEs)ELISA Kit
GA-E0614RT-48T 48T
EUR 380.4

Rat advanced glycation end products(AGEs)ELISA Kit
GA-E0614RT-96T 96T
EUR 595.2

Rat advanced glycation end products, AGEs ELISA Kit
ELA-E0263r 96 Tests
EUR 1063.2

Rat advanced glycation end products(AGEs)ELISA Kit
QY-E11038 96T
EUR 433.2

Rat advanced glycation end products,AGEs ELISA Kit
YLA0170RA-48T 48T
EUR 465

Rat advanced glycation end products,AGEs ELISA Kit
YLA0170RA-96T 96T
EUR 600

Human advanced Glycation end product (AGE) Elisa kit
YLA4301HU-48T 48T
EUR 435

Human advanced Glycation end product (AGE) Elisa kit
YLA4301HU-96T 96T
EUR 562.5

Human advanced glycation end products,AGEs ELISA Kit
201-12-0004 96 tests
EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
CEB353Ge-10x96wellstestplate 10x96-wells test plate
EUR 5764.2
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
CEB353Ge-1x48wellstestplate 1x48-wells test plate
EUR 579.88
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
CEB353Ge-1x96wellstestplate 1x96-wells test plate
EUR 776.96
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
CEB353Ge-5x96wellstestplate 5x96-wells test plate
EUR 3136.34
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of General Advanced Glycation End Product (AGE) in serum, plasma and other biological fluids.

General Advanced Glycation End Product (AGE) ELISA Kit
4-CEB353Ge
  • EUR 5824.80
  • EUR 3076.80
  • EUR 777.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of General Advanced Glycation End Product (AGE) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse advanced glycation end products,AGEs ELISA Kit
CN-02549M1 96T
EUR 565.2

Mouse advanced glycation end products,AGEs ELISA Kit
CN-02549M2 48T
EUR 386.4

Human advanced glycation end products,AGEs ELISA Kit
CN-04128H1 96T
EUR 567.6

Human advanced glycation end products,AGEs ELISA Kit
CN-04128H2 48T
EUR 387.6

Human advanced glycation end products, AGEs ELISA Kit
CSB-E09412h-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Human advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human advanced glycation end products, AGEs ELISA Kit
1-CSB-E09412h
  • EUR 1080.00
  • EUR 6571.20
  • EUR 3480.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse advanced glycation end products, AGEs ELISA Kit
CSB-E09414m-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Mouse advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Mouse advanced glycation end products, AGEs ELISA Kit
1-CSB-E09414m
  • EUR 1135.20
  • EUR 6938.40
  • EUR 3672.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Mouse advanced glycation end products, AGEs in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Mouse advanced glycation end products, AGEs ELISA Kit
E0263m 96 Tests
EUR 813.6

Mouse AGE/ Advanced glycation end-product ELISA Kit
E0051Mo 1 Kit
EUR 685.2

Human AGE/ Advanced glycation end-product ELISA Kit
E0080Hu 1 Kit
EUR 685.2

Mouse advanced glycation end products(AGEs)ELISA Kit
GA-E0602MS-48T 48T
EUR 403.2

Mouse advanced glycation end products(AGEs)ELISA Kit
GA-E0602MS-96T 96T
EUR 640.8

Human advanced glycation end products(AGEs)ELISA Kit
GA-E0051HM-48T 48T
EUR 346.8

Human advanced glycation end products(AGEs)ELISA Kit
GA-E0051HM-96T 96T
EUR 559.2

Human AGE(Advanced glycation end-product) ELISA Kit
EH0622 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.188 ng/ml

ELISA kit for Mouse Advanced glycation end-product
EK5086 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Mouse Advanced glycation end-product in samples from serum, plasma, tissue homogenates and other biological fluids.

General Advanced Glycation End Product ELISA Kit (AGE)
RK00619 96 Tests
EUR 625.2

Mouse advanced glycation end products(AGEs)ELISA Kit
QY-E20056 96T
EUR 433.2

Human advanced glycation end products(AGEs)ELISA Kit
QY-E01118 96T
EUR 433.2

Mouse advanced glycation end products,AGEs ELISA Kit
YLA0359MO-48T 48T
EUR 465

Mouse advanced glycation end products,AGEs ELISA Kit
YLA0359MO-96T 96T
EUR 600

Mouse advanced glycation end products,AGEs ELISA Kit
YLA0180MO-48T 48T
EUR 465

Mouse advanced glycation end products,AGEs ELISA Kit
YLA0180MO-96T 96T
EUR 600

Human advanced glycation end products,AGEs ELISA Kit
YLA1489HU-48T 48T
EUR 435

Human advanced glycation end products,AGEs ELISA Kit
YLA1489HU-96T 96T
EUR 562.5

ELISA kit for Rat Advanced glycation end products (AGEs)
KTE100353-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Rat Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Advanced glycation end products (AGEs)
KTE100353-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Rat Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Rat Advanced glycation end products (AGEs)
KTE100353-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Rat Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Advanced glycation end products (AGEs)
KTE60961-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Human Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Advanced glycation end products (AGEs)
KTE60961-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Human Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Human Advanced glycation end products (AGEs)
KTE60961-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Human Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Advanced glycation end products (AGEs)
KTE70569-48T 48T
EUR 424.8
Description: Quantitative sandwich ELISA for measuring Mouse Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Advanced glycation end products (AGEs)
KTE70569-5platesof96wells 5 plates of 96 wells
EUR 2702.4
Description: Quantitative sandwich ELISA for measuring Mouse Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

ELISA kit for Mouse Advanced glycation end products (AGEs)
KTE70569-96T 96T
EUR 686.4
Description: Quantitative sandwich ELISA for measuring Mouse Advanced glycation end products (AGEs) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.

Mouse AGE(Mouse advanced glycation end product) ELISA Kit
EM0716 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.094 ng/ml

ELISA kit for General AGE (Advanced Glycation End Product)
ELK1890 1 plate of 96 wells
EUR 446.4
Description: A competitive Inhibition ELISA kit for detection of Advanced Glycation End Product from General in samples from blood, serum, plasma, cell culture fluid and other biological fluids.

Guinea Pig advanced glycation end products,AGEs ELISA Kit
CN-00256G1 96T
EUR 565.2

Improved HPLC Conditions to Determine Eumelanin and Pheomelanin Contents in Biological Samples Using an Ion Pair Reagent

Alkaline hydrogen peroxide oxidation (AHPO) of eumelanin and pheomelanin, two major classes of melanin pigments, affords pyrrole-2,3,5-tricarboxylic acid (PTCA), pyrrole-2,3-dicarboxylic acid (PDCA) and pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA) from eumelanin and thiazole-2,4,5-tricarboxylic acid (TTCA) and thiazole-4,5-dicarboxylic acid (TDCA) from pheomelanin.
Quantification of these five markers by HPLC provides useful information on the quantity and structural diversity of melanins in various biological samples. HPLC analysis of these markers using the original method of 0.1 M potassium phosphate buffer (pH 2.1):methanol = 99:1 (85:15 for PTeCA) on a reversed-phase column had some problems, including the short lifetime of the column and, except for the major eumelanin marker PTCA, other markers were occasionally overlapped by interfering peaks in samples containing only trace levels of these markers.
These problems can be overcome by the addition of an ion pair reagent for anions, such as tetra-n-butylammonium bromide (1 mM), to retard the elution of di-, tri- and tetra-carboxylic acids. The methanol concentration was increased to 17% (30% for PTeCA) and the linearity, reproducibility, and recovery of the markers with this improved method is good to excellent.
 This improved HPLC method was compared to the original method using synthetic melanins, mouse hair, human hair, and human epidermal samples. In addition to PTCA, TTCA, a major marker for pheomelanin, showed excellent correlations between both HPLC methods. The other markers showed an attenuation of the interfering peaks with the improved method. We recommend this improved HPLC method for the quantitative analysis of melanin markers following AHPO because of its simplicity, accuracy, and reproducibility.
Keywords: 4-amino-3-hydroxyphenylalanine (4-AHP); alkaline hydrogen peroxide oxidation (AHPO); high-performance liquid chromatography (HPLC); individual typology angle (ITA); pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA); pyrrole-2,3,5-tricarboxylic acid (PTCA); pyrrole-2,3-dicarboxylic acid (PDCA); thiazole-2,4,5-tricarboxylic acid (TTCA); thiazole-4,5-dicarboxylic acid (TDCA); total melanin (TM).

Manipulating the click reactivity of dibenzoazacyclooctynes: from an azide clicker to a caged acylation reagent

Strain-promoted azide-alkyne cycloaddition using dibenzoazacyclooctyne (DBCO) is widely applied in copper-free bioorthogonal reactions. Here, we report the efficient acid-promoted rearrangement and silver-catalyzed amidation of DBCO, which alters its click reactivity robustly. In the switched click reaction, DBCO, as a caged acylation reagent, enables rapid peptide/protein modification after decaged by silver catalysts that renders site-specific conjugation of IgG antibody via an Fc-targeting peptide.
Keywords: DBCO; caged acylation reagent; click chemistry; silver catalyst; switched click reaction.

Rabbit control serum (non-immunized, Disease free, SPF Rabbits)
NSPF-5 5 ml
EUR 634.8

Human IgG-Biotin (serum origin, purified >97%, low endotoxin, azide free)
20007-1-LE-BTN 100 ug
EUR 343.2

Steroid Free Serum
90R-1007 50 mL
EUR 470.4
Description: Steroid Free Human Serum

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-100 each
EUR 783.6

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-25 each
EUR 294

Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0
7917-5 each
EUR 144

Albumin Depletion Reagent for Plasma and Serum (20 ml)
P5W13 - Ask for price

EZ-DNA Reagents
BS8202 100preps
EUR 105.67

EZ-DNA Reagents
SK8201 100preps
EUR 112.2

Ecl Detection Reagents
RPN2105 EACH
EUR 724.8

Ecl Detection Reagents
RPN3004 EACH
EUR 582

Human Serum (CRP free)
90R-100 100 ml
EUR 1326
Description: C-reactive protein free normal human serum

Human Serum (FABP free)
90R-109 100 ml
EUR 1326
Description: FABP free normal human serum

Rabbit Serum
abx098265-100ml 100 ml
EUR 326.4

Rabbit Serum
abx098266-500ml 500 ml
EUR 577.2

Rabbit Serum
1267-1 each
EUR 188.4

REAGENTS HIGH RANGE AMMONIA
HI73325 PK25
EUR 49.2

High Range Ammonia Reagents
HI9373301 EACH
EUR 116.4

Human Serum (Myoglobin Free)
90R-110 100 ml
EUR 1326
Description: Myoglobin free normal human serum

CryoProtX Mix Reagents 1.5 mL
M-MDSR-61 1.5 ml ml
EUR 37
Description: CryoProtX Mix Reagents 1.5 mL

Alkphos Direct Labelling Reagents
RPN3680 EACH
EUR 813.6

Set of 10 Biolipidure Reagents
Biolipidure-set 10mLx10
EUR 1820.4
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement

Human Serum (Troponin I Free)
90R-106 100 ml
EUR 1417.2
Description: Troponion I free normal human serum

Rabbit Serum S TH 100mL
31126-1 100mL
EUR 211.12

Rabbit Serum S TH 500mL
31126-5 500mL
EUR 276.36

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-1 1 mg
EUR 169.2

Rabbit IgG, purified (serum non-immune, isotype control), without azide, powder
20009-WA-5 5 mg
EUR 343.2

CryoProtX Mix Eco Reagents 1.5 mL
M-MDSR-61-ECO 1.5 ml ml
EUR 37
Description: CryoProtX Mix Eco Reagents 1.5 mL

500mL INSECTAGRO SF9 serum free
13-410-CV PK6
EUR 222

Normal Rabbit Serum
88-NR50 100 ml
EUR 180
Description: Normal Rabbit Serum

Normal Rabbit Serum
88R-1004 10 ml
EUR 247.2
Description: Normal Rabbit Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Normal Rabbit Serum
89R-RM001 20 mg
EUR 470.4
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum
89R-RM002 5 ml
EUR 289.2
Description: Normal Rabbit Serum (Mouse cell adsorbed)

Normal Rabbit Serum
89R-RR001 20 mg
EUR 418.8
Description: Normal Rabbit Serum (Rat cell adsorbed)

Normal Rabbit Serum
89R-RR002 5 ml
EUR 289.2
Description: Normal Rabbit Serum (Rat cell adsorbed)

Serum Albumin, Lipid Free Protein
20-abx260049
  • EUR 927.60
  • EUR 343.20
  • EUR 627.60
  • 100 mg
  • 10 mg
  • 50 mg

Rabbit Serum Albumin
30R-3303 10 mg
EUR 224.4
Description: Purified native Rabbit Serum Albumin

Rabbit Serum (Sterile)
20-abx800002
  • EUR 1680.00
  • EUR 3951.60
  • EUR 994.80
  • 100 ml
  • 500 ml
  • 50 ml

Rabbit Serum (Sterile)
abx800002-1L 1 L
EUR 6524.4

Bovine Serum Albumin, protease free
GK4012-1KG 1 kg
EUR 1165.2

Bovine Serum Albumin, protease free
GK4012-500G 500 g
EUR 638.4

Bovine Serum Albumin, protease free
GK4012-1 1
EUR 2065.2

Bovine Serum Albumin, protease free
GK4012-500 500
EUR 1181.9

Rabbit Serum NS TH MT 500mL
31116-5 500mL
EUR 235.91

Rabbit Serum NS NH YG 100mL
31118-1 100mL
EUR 211.12

Rabbit Serum NS NH YG 500mL
31118-5 500mL
EUR 306.37

Rabbit Serum NS TH YG 100mL
31119-1 100mL
EUR 186.31

Rabbit Serum NS TH YG 500mL
31119-5 500mL
EUR 267.23

Rabbit Serum S NH YG 100ml
31125-1 100mL
EUR 228.07

Rabbit Serum S NH YG 500ml
31125-5 500mL
EUR 333.78

Rabbit Serum NH S HI YG 500mL
31127-5 500mL
EUR 371.63

Rabbit Serum S TH YG HI 500mL
31128-5 500mL
EUR 306.37

Bovine Serum Albumin, fatty acid free
GX5685-1KG 1 kg
EUR 1442.4

Bovine Serum Albumin, fatty acid free
GX5685-500G 500 g
EUR 792

Bovine Serum Albumin, fatty acid free
GX5685-1 1
EUR 1162.8

Bovine Serum Albumin, fatty acid free
GX5685-500 500
EUR 624.9

Rabbit Complement Serum
32R-CR001 25 ml
EUR 289.2
Description: Purifed Rabbit Complement isolated from blood of New Zealand white rabbits

Normal Bovine Serum (Protease/IgG free)
88R-1012 10 ml
EUR 218.4
Description: Normal Bovine Serum which has been lipid extracted and dialyzed against 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Rabbit anti Pig Serum
40R-RS001 2 ml
EUR 289.2
Description: Rabbit anti Pig Serum antibody

Human Apolipoprotein AI / B Free Serum Protein
abx060869-100ml 100 ml
EUR 594

Rabbit Serum (Non-Sterile
20-abx800001
  • EUR 1296.00
  • EUR 2966.40
  • EUR 794.40
  • 100 ml
  • 500 ml
  • 50 ml

Rabbit Serum (Non-Sterile
abx800001-1L 1 L
EUR 5137.2

Non-sterile rabbit serum
RS05-0100 100 ml
EUR 199.68

Non-sterile rabbit serum
RS05-0500 500ml
EUR 230.88

Rabbit Serum NS NH YG 3L
31118-3 3L
EUR 1148.1

Rabbit Serum NS TH YG 3L
31119-3 3L
EUR 1039.79

Bradford(Coomassie) Protein Assay Plus Reagents
P7201-050 450ml
EUR 225.6

Rabbit Serum Iron ELISA kit
E04S0273-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Iron ELISA kit
E04S0273-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Iron ELISA kit
E04S0273-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Iron in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Corning Lymphocyte Serum Free Medium KBM551
88-551-CM 1L
EUR 201.6

Corning Lymphocyte Serum Free Medium KBM581
88-581-CM 1L
EUR 266.4

Fetal Bovine Serum, Premium Tetracyclin Free
F0500-050 500ml
EUR 1388.4

Rabbit Serum Albumin ELISA kit
E04S0014-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Albumin ELISA kit
E04S0014-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum Albumin ELISA kit
E04S0014-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Serum Albumin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit
E04S0228-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit
E04S0228-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Serum amyloid ELISA kit
E04S0228-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Serum amyloid in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pre-immune normal rabbit serum
NRS01 200 µl
EUR 358.8

Serum Free Medium For Rat Neural Stem Cells
RAXNF-90011 100mL
EUR 289

Rabbit Serum NH S DELIPID. 500mL
31117-5 500mL
EUR 358.57

Rabbit Serum amyloid A ELISA Kit
ELA-E0885Rb 96 Tests
EUR 1113.6

Serum Free Medium For Mouse Neural Stem Cells
MUXNF-90011 100mL
EUR 289

Rabbit Serum albumin, ALB ELISA KIT
ELI-03306Rb 96 Tests
EUR 1113.6
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