WordPress database error: [Unknown column 'main_image' in 'field list']
SELECT
id,
product_name,
product_catalog_number,
supplier_id,
supplier_name,
product_detail_size,
product_detail_price_buy,
product_detail_price_sell,
product_detail_description,
product_detail_specifications,
product_detail_additional_information,
product_detail_storage_and_shipping,
product_detail_notes,
product_type,
main_image
FROM gentaur_products
WHERE id IN ("Abbexa|abx102967-100l","Abbexa|abx102967-1ml","Abbexa|abx102967-200l","Abbexa|20-abx273553","Abbexa|20-abx271739","Biomatik Corporation|CAU29357-100ul","Biomatik Corporation|CAU29357-200ul","SAB|AP79793","SAB|AP80037","SAB|AP80048","Cloud-Clone|MAB353Ge21","Cloud-Clone|PAB353Ge01","MyBiosource|MBS2025667-INQUIRE","GenWay Biotech|GWB-B07BB8","Cloud-Clone|4-MAB353Ge21","Cloud-Clone|4-PAB353Ge01","Cloud-Clone|4-MAB353Ge21-PE","Cloud-Clone|4-PAB353Ge01-PE","Cloud-Clone|4-MAB353Ge21-APC","Cloud-Clone|4-MAB353Ge21-Cy3","Cloud-Clone|4-MAB353Ge21-FITC","Cloud-Clone|4-MAB353Ge21-HRP","Cloud-Clone|4-PAB353Ge01-APC","Cloud-Clone|4-PAB353Ge01-Cy3","Cloud-Clone|4-PAB353Ge01-FITC","Cloud-Clone|4-PAB353Ge01-HRP","GenWay Biotech|GWB-9FDA8E","Kamiya Biomedical Company|KT-6025","Genovis AB|ECP7801","Cloud-Clone|4-APB353Ge01","Cloud-Clone|NPB353Ge01","Cloud-Clone|4-NPB353Ge01","Biomatik Corporation|RPU54925-100ug","Biomatik Corporation|RPU54925-1mg","Biomatik Corporation|RPU54925-50ug","Biomatik Corporation|RPU50405-100ug","Biomatik Corporation|RPU50405-1mg","Biomatik Corporation|RPU50405-50ug","MyBiosource|MBS2018775-001mg","MyBiosource|MBS2018775-005mg","MyBiosource|MBS2018775-01mg","MyBiosource|MBS2018775-02mg","MyBiosource|MBS2018775-05mg","MyBiosource|MBS2097354-001mg","MyBiosource|MBS2097354-005mg","MyBiosource|MBS2097354-01mg","MyBiosource|MBS2097354-02mg","MyBiosource|MBS2097354-05mg","Abbexa|20-abx168580","Abbexa|abx168580-1ml","MyBiosource|MBS2054212-INQUIRE","MyBiosource|MBS2054214-INQUIRE","MyBiosource|MBS2054216-INQUIRE","MyBiosource|MBS2054219-INQUIRE","Cloud-Clone|4-MAB353Ge21-APC-Cy7","Cloud-Clone|4-PAB353Ge01-APC-Cy7","MyBiosource|MBS2054217-INQUIRE","Cloud-Clone|4-MAB353Ge21-Biotin","Cloud-Clone|4-PAB353Ge01-Biotin","Abbexa|20-abx490411","MyBiosource|MBS2091058-INQUIRE","Abbexa|20-abx150316","Abbexa|abx255064-96tests","Abbexa|abx576530-96tests","DL Develop|DLR-AGE-Ge-48T","DL Develop|DLR-AGE-Ge-96T","Biomatik Corporation|EKN43304-48T","Biomatik Corporation|EKN43304-5x96T","Biomatik Corporation|EKN43304-96T","Biomatik Corporation|EKU02193-48T","Biomatik Corporation|EKU02193-5x96T","Biomatik Corporation|EKU02193-96T","Biomatik Corporation|EKU10908-48T","Biomatik Corporation|EKU10908-5x96T","Biomatik Corporation|EKU10908-96T","Biomatik Corporation|EKN52019-48T","Biomatik Corporation|EKN52019-5x96T","Biomatik Corporation|EKN52019-96T","ELK Biotech|ELK1890-48T","ELK Biotech|ELK1890-96T","Abbexa|abx054078-100g","Abbexa|abx054078-10g","Abbexa|abx054078-50g","BlueGene|E01A16262","MyBiosource|MBS2054210-INQUIRE","MyBiosource|MBS2100249-10x96Tests","MyBiosource|MBS2100249-10x96TestsMBS2090686AbPairsSupportPack210x96Tests","MyBiosource|MBS2100249-5x96Tests","MyBiosource|MBS2100249-5x96TestsMBS2090686AbPairsSupportPack25x96Tests","BlueGene|E01A16586","Biovision|K929-100","MyBiosource|MBS9425430-5x96Tests","MyBiosource|MBS9425430-96Tests","MyBiosource|MBS7608032-10x96StripWells","MyBiosource|MBS7608032-48StripWells");
WordPress database error: [Unknown column 'main_image' in 'field list']
SELECT
id,
product_name,
product_catalog_number,
supplier_id,
supplier_name,
product_detail_size,
product_detail_price_buy,
product_detail_price_sell,
product_detail_description,
product_detail_specifications,
product_detail_additional_information,
product_detail_storage_and_shipping,
product_detail_notes,
product_type,
main_image
FROM gentaur_products
WHERE id IN ("GenWay Biotech|GWB-Q00010","GenWay Biotech|GWB-Q00011","GenWay Biotech|GWB-Q00012","GenWay Biotech|GWB-Q00013","GenWay Biotech|GWB-Q00014","GenWay Biotech|GWB-Q00005","Aviva Systems Biology|OOSA10411-5ML","Aviva Systems Biology|OOSA10417-50ML","Aviva Systems Biology|OOSA10426-20ML","Aviva Systems Biology|OOSA10430-10ML","Aviva Systems Biology|OOSA10419-100ML","Aviva Systems Biology|OOSA11794-100ML","MyBiosource|MBS239301-100mL","MyBiosource|MBS239301-5x100mL","Aviva Systems Biology|OOSA10413-5ML","Aviva Systems Biology|OOSA10415-10ML","Aviva Systems Biology|OOSA10428-100ML","SBI|EXOFLOW2-BASICA-SP","GenWay Biotech|GWB-F06E29","GenWay Biotech|GWB-DCC190","Scientific Laboratory Supplies|WAT1028","GenWay Biotech|GWB-Q00017","GenWay Biotech|GWB-Q00018","MyBiosource|MBS6120697-025mg","MyBiosource|MBS6120697-1mg","MyBiosource|MBS6120697-5x1mg","Vazyme|R602","MyBiosource|MBS568775-100g","MyBiosource|MBS568775-5x100g","Bio Basic|BS8202","Bio Basic|SK8201","Scientific Laboratory Supplies|RPN3004","MyBiosource|MBS6121446-02mL","MyBiosource|MBS6121446-5x02mL","Biovision|7917-100","Biovision|7917-25","Biovision|7917-5","T-Pro Biotechnology|JO66-V002M","T-Pro Biotechnology|JO66-V002S","MyBiosource|MBS6120698-025mg","MyBiosource|MBS6120698-1mg","MyBiosource|MBS6120698-5x1mg","101Bio|P5W13","Creative Bioarray|CEIK-044L-30T","Creative Bioarray|CEIK-044L-50T","MyBiosource|MBS6120696-1mg","MyBiosource|MBS6120696-5x1mg","Scientific Laboratory Supplies|RPN2105","Aviva Systems Biology|OPEF01351-100G","Inventbiotech|WA-013","MiTeGen|M-MDSR-61","AS ONE International|Biolipidure-set","Scientific Laboratory Supplies|HI73325","Scientific Laboratory Supplies|HI9373301","MiTeGen|M-MDSR-61-ECO","Sisco Laboratories|65186","MyBiosource|MBS6491998-02mL","MyBiosource|MBS6491998-5x02mL","Tecan Benelux|M1980","Scientific Laboratory Supplies|RPN3680","Alpha Diagnostics|20007-1-LE-1","Alpha Diagnostics|NSPF-1","Alpha Diagnostics|NSPF-10","Alpha Diagnostics|NSPF-5","MyBiosource|MBS6344814-02mL","MyBiosource|MBS6344814-5x02mL","GenDepot|P7201-050","GenDepot|P7201-100","MyBiosource|MBS6335888-02mL","MyBiosource|MBS6335888-5x02mL","MyBiosource|MBS6335899-02mL","MyBiosource|MBS6335899-5x02mL","MyBiosource|MBS590076-10Plates","MyBiosource|MBS590076-5Plates","MyBiosource|MBS590076-5x10Plates","MyBiosource|MBS238207-20mL","MyBiosource|MBS238207-5x20mL","MyBiosource|MBS238208-100mL","MyBiosource|MBS238208-5x100mL","GenWay Biotech|GWB-FD777C","MyBiosource|MBS663102-1Unit","MyBiosource|MBS663102-5x1Unit","MyBiosource|MBS318434-50mL","MyBiosource|MBS318434-5x50mL","Abbexa|abx298032-100g");
Atmospheric Pressure Plasma Jet Treatment of Polymers Enables Reagent-Free Covalent Attachment of Biomolecules for Bioprinting
3D bioprinting, where cells, hydrogels and structural polymers can be printed layer by layer into complex designs, holds great promise for advances in medicine and the biomedical sciences. In principle, this technique enables the creation of highly patient specific disease models and biomedical implants. However, an ability to tailor surface biocompatibility and interfacial bonding between printed components, such as polymers and hydrogels, is currently lacking.
- Here we demonstrate that an atmospheric pressure plasma jet (APPJ) can locally activate polymeric surfaces for the reagent-free covalent attachment of proteins and hydrogel in a single-step process at desired locations. Polyethylene and poly--caprolactone (PCL) were used as example polymers. Covalent attachment of the proteins and hydrogel was demonstrated by resistance to removal by rigorous sodium dodecyl sulfate (SDS) washing.
- The immobilized protein and hydrogel layers were analyzed using Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS). Importantly, the APPJ surface activation also rendered the polymer surfaces mildly hydrophilic as required for optimum biocompatibility.
- Water contact angles were observed to be stable within a range where the conformation of biomolecules is preserved. Single and double electrode designs of APPJs were compared in their characteristics relevant to localized surface functionalization, plume length and shape. As a proof of efficacy in a biological context, APPJ treated polyethylene functionalized with fibronectin was used to demonstrate improvements in cell adhesion and proliferation.
- These results have important implications for the development of a new generation of 3D bioprinters capable of spatially patterned and tailored surface functionalization performed during the 3D printing process in situ.
gentaur.com
Advanced Glycation End Product (AGE) Antibody |
abx102967-100l |
Abbexa |
100 µl |
EUR 287.5 |
Advanced Glycation End Product (AGE) Antibody |
abx102967-1ml |
Abbexa |
1 ml |
EUR 850 |
Advanced Glycation End Product (AGE) Antibody |
abx102967-200l |
Abbexa |
200 µl |
EUR 375 |
Advanced Glycation End Product (AGE) Antibody (FITC) |
20-abx273553 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Advanced Glycation End Product (AGE) Antibody (Biotin) |
20-abx271739 |
Abbexa |
-
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Advanced Glycation End Product (AGE) Monoclonal Antibody |
CAU29357-100ul |
Biomatik Corporation |
100ul |
EUR 267.8 |
Advanced Glycation End Product (AGE) Monoclonal Antibody |
CAU29357-200ul |
Biomatik Corporation |
200ul |
EUR 334.3 |
Advanced glycation end-product |
AP79793 |
SAB |
1mg |
EUR 2640 |
|
Advanced glycation end-product |
AP80037 |
SAB |
1mg |
EUR 2640 |
|
Advanced glycation end-product |
AP80048 |
SAB |
1mg |
EUR 2640 |
|
Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MAB353Ge21 |
Cloud-Clone |
100ul |
EUR 279 |
|
Polyclonal Antibody to Advanced Glycation End Product (AGE) |
PAB353Ge01 |
Cloud-Clone |
100ul |
EUR 279 |
|
Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MBS2025667-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
Anti- Advanced Glycation End-product (AGE) Antiserum Antibody |
GWB-B07BB8 |
GenWay Biotech |
0.25 ml |
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Advanced Glycation End Product (AGE) Monoclonal Antibody (General) |
4-MAB353Ge21 |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE) |
Advanced Glycation End Product (AGE) Polyclonal Antibody (General) |
4-PAB353Ge01 |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE) |
Advanced Glycation End Product (AGE) Monoclonal Antibody (General), PE |
4-MAB353Ge21-PE |
Cloud-Clone |
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|
- 100ul
- 10ml
- 1ml
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|
|
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with PE. |
Advanced Glycation End Product (AGE) Polyclonal Antibody (General), PE |
4-PAB353Ge01-PE |
Cloud-Clone |
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with PE. |
Advanced Glycation End Product (AGE) Monoclonal Antibody (General), APC |
4-MAB353Ge21-APC |
Cloud-Clone |
-
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- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC. |
Advanced Glycation End Product (AGE) Monoclonal Antibody (General), Cy3 |
4-MAB353Ge21-Cy3 |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Cy3. |
Advanced Glycation End Product (AGE) Monoclonal Antibody (General), FITC |
4-MAB353Ge21-FITC |
Cloud-Clone |
-
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- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with FITC. |
Advanced Glycation End Product (AGE) Monoclonal Antibody (General), HRP |
4-MAB353Ge21-HRP |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with HRP. |
Advanced Glycation End Product (AGE) Polyclonal Antibody (General), APC |
4-PAB353Ge01-APC |
Cloud-Clone |
-
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-
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-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC. |
Advanced Glycation End Product (AGE) Polyclonal Antibody (General), Cy3 |
4-PAB353Ge01-Cy3 |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Cy3. |
Advanced Glycation End Product (AGE) Polyclonal Antibody (General), FITC |
4-PAB353Ge01-FITC |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with FITC. |
Advanced Glycation End Product (AGE) Polyclonal Antibody (General), HRP |
4-PAB353Ge01-HRP |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with HRP. |
Advanced Glycation End Product (AGE) -BSA |
GWB-9FDA8E |
GenWay Biotech |
10 mg |
Ask for price |
Advanced Glycation End Product ELISA Kit |
ECP7801 |
Genovis AB |
96 Tests |
EUR 563 |
Active Advanced Glycation End Product (AGE) |
4-APB353Ge01 |
Cloud-Clone |
-
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|
- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Pan-species Advanced Glycation End Product expressed in: Human |
Native Advanced Glycation End Product (AGE) |
NPB353Ge01 |
Cloud-Clone |
10ug |
EUR 120 |
Native Advanced Glycation End Product (AGE) |
4-NPB353Ge01 |
Cloud-Clone |
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- 100 ug
- 10ug
- 1 mg
- 200 ug
- 500 ug
- 50ug
- 5 mg
|
|
Description: Recombinant Pan-species Advanced Glycation End Product expressed in: Natural extract |
Native Advanced Glycation End Product (AGE) |
MBS2018775-001mg |
MyBiosource |
0.01mg |
EUR 140 |
Native Advanced Glycation End Product (AGE) |
MBS2018775-005mg |
MyBiosource |
0.05mg |
EUR 235 |
Native Advanced Glycation End Product (AGE) |
MBS2018775-01mg |
MyBiosource |
0.1mg |
EUR 325 |
Native Advanced Glycation End Product (AGE) |
MBS2018775-02mg |
MyBiosource |
0.2mg |
EUR 400 |
Native Advanced Glycation End Product (AGE) |
MBS2018775-05mg |
MyBiosource |
0.5mg |
EUR 745 |
Active Advanced Glycation End Product (AGE) |
MBS2097354-001mg |
MyBiosource |
0.01mg |
EUR 165 |
Active Advanced Glycation End Product (AGE) |
MBS2097354-005mg |
MyBiosource |
0.05mg |
EUR 285 |
Active Advanced Glycation End Product (AGE) |
MBS2097354-01mg |
MyBiosource |
0.1mg |
EUR 425 |
Active Advanced Glycation End Product (AGE) |
MBS2097354-02mg |
MyBiosource |
0.2mg |
EUR 515 |
Active Advanced Glycation End Product (AGE) |
MBS2097354-05mg |
MyBiosource |
0.5mg |
EUR 980 |
Advanced Glycation End Product (AGE) Protein |
20-abx168580 |
Abbexa |
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|
- 100 ug
- 10 ug
- 1 mg
- 200 ug
- 50 ug
|
|
Advanced Glycation End Product (AGE) Protein |
abx168580-1ml |
Abbexa |
1 ml |
EUR 300 |
PE-Linked Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MBS2054212-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
APC-Linked Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MBS2054214-INQUIRE |
MyBiosource |
INQUIRE |
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Cy3-Linked Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MBS2054216-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
HRP-Linked Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MBS2054219-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
Advanced Glycation End Product (AGE) Monoclonal Antibody (General), APC-Cy7 |
4-MAB353Ge21-APC-Cy7 |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC-Cy7. |
Advanced Glycation End Product (AGE) Polyclonal Antibody (General), APC-Cy7 |
4-PAB353Ge01-APC-Cy7 |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with APC-Cy7. |
FITC-Linked Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MBS2054217-INQUIRE |
MyBiosource |
INQUIRE |
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Advanced Glycation End Product (AGE) Monoclonal Antibody (General), Biotinylated |
4-MAB353Ge21-Biotin |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Mouse monoclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Biotin. |
Advanced Glycation End Product (AGE) Polyclonal Antibody (General), Biotinylated |
4-PAB353Ge01-Biotin |
Cloud-Clone |
-
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|
- 100ul
- 10ml
- 1ml
- 200ul
- 20ul
|
|
Description: A Rabbit polyclonal antibody against General Advanced Glycation End Product (AGE). This antibody is labeled with Biotin. |
Advanced Glycation End Product (AGE) CLIA Kit |
20-abx490411 |
Abbexa |
-
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|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Biotin-Linked Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MBS2091058-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
Advanced Glycation End Product (AGE) ELISA Kit |
20-abx150316 |
Abbexa |
-
Ask for price
-
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-
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|
- 10 × 96 tests
- 5 × 96 tests
- 96 tests
|
|
Advanced Glycation End Product (AGE) ELISA Kit |
abx255064-96tests |
Abbexa |
96 tests |
EUR 895.2 |
|
Advanced Glycation End Product (AGE) ELISA Kit |
abx576530-96tests |
Abbexa |
96 tests |
EUR 895.2 |
|
Advanced Glycation End Product (AGE) ELISA Kit |
DLR-AGE-Ge-48T |
DL Develop |
48T |
EUR 627.6 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Advanced Glycation End Product (AGE) ELISA Kit |
DLR-AGE-Ge-96T |
DL Develop |
96T |
EUR 817.2 |
|
Description: A competitive inhibition quantitative ELISA assay kit for detection of Advanced Glycation End Product (AGE) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Advanced Glycation End Product (AGE) ELISA Kit |
EKN43304-5x96T |
Biomatik Corporation |
5x96T |
EUR 2612.03 |
Advanced Glycation End Product (AGE) ELISA Kit |
EKU10908-5x96T |
Biomatik Corporation |
5x96T |
EUR 4211.83 |
Advanced Glycation End Product (AGE) ELISA Kit |
EKN52019-5x96T |
Biomatik Corporation |
5x96T |
EUR 2621.05 |
AGE(Advanced Glycation End Product) ELISA Kit |
ELK1890-48T |
ELK Biotech |
48T |
Ask for price |
|
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGE in the samples is then determined by comparing the OD of the samples to the standard curve. |
AGE(Advanced Glycation End Product) ELISA Kit |
ELK1890-96T |
ELK Biotech |
96T |
Ask for price |
|
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with AGE protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to AGE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of AGE in the samples is then determined by comparing the OD of the samples to the standard curve. |
Advanced Glycation End Product (AGE) ELISA Kit |
abx054078-100g |
Abbexa |
100 µg |
Ask for price |
Advanced Glycation End Product (AGE) ELISA Kit |
abx054078-10g |
Abbexa |
10 µg |
EUR 556.25 |
Advanced Glycation End Product (AGE) ELISA Kit |
abx054078-50g |
Abbexa |
50 µg |
Ask for price |
Rat Receptor for advanced glycatiom end products/Advanced Glycosylation End Product Specific Receptor ELISA kit |
E01A16262 |
BlueGene |
96T |
EUR 700 |
Description: ELISA |
APC/CY7-Linked Monoclonal Antibody to Advanced Glycation End Product (AGE) |
MBS2054210-INQUIRE |
MyBiosource |
INQUIRE |
Ask for price |
General Advanced Glycation End Product (AGE) Antibody Pair Kit (with Standard) |
MBS2100249-10x96Tests |
MyBiosource |
10x96Tests |
EUR 1780 |
General Advanced Glycation End Product (AGE) Antibody Pair Kit (with Standard) |
MBS2100249-10x96TestsMBS2090686AbPairsSupportPack210x96Tests |
MyBiosource |
10x96Tests+MBS2090686(AbPairsSupportPack2,10x96Tests) |
EUR 1900 |
General Advanced Glycation End Product (AGE) Antibody Pair Kit (with Standard) |
MBS2100249-5x96Tests |
MyBiosource |
5x96Tests |
EUR 1115 |
General Advanced Glycation End Product (AGE) Antibody Pair Kit (with Standard) |
MBS2100249-5x96TestsMBS2090686AbPairsSupportPack25x96Tests |
MyBiosource |
5x96Tests+MBS2090686(AbPairsSupportPack2,5x96Tests) |
EUR 1185 |
Rat Solube receptor for advanced glycation end products/Endogenous secretory receptor for Advanced Glycation end Products ELisa Kit |
E01A16586 |
BlueGene |
96T |
EUR 700 |
Description: ELISA |
Advanced Glycation End Products (AGEs) Assay Kit |
K929-100 |
Biovision |
each |
EUR 502.8 |
Rat Advanced glycation end-product ELISA Kit |
MBS9425430-5x96Tests |
MyBiosource |
5x96Tests |
EUR 2820 |
Rat Advanced glycation end-product ELISA Kit |
MBS9425430-96Tests |
MyBiosource |
96Tests |
EUR 615 |
AGE (Advanced glycation end-product) ELISA Kit |
MBS7608032-10x96StripWells |
MyBiosource |
10x96-Strip-Wells |
EUR 3900 |
AGE (Advanced glycation end-product) ELISA Kit |
MBS7608032-48StripWells |
MyBiosource |
48-Strip-Wells |
EUR 340 |
Improved HPLC Conditions to Determine Eumelanin and Pheomelanin Contents in Biological Samples Using an Ion Pair Reagent
Alkaline hydrogen peroxide oxidation (AHPO) of eumelanin and pheomelanin, two major classes of melanin pigments, affords pyrrole-2,3,5-tricarboxylic acid (PTCA), pyrrole-2,3-dicarboxylic acid (PDCA) and pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA) from eumelanin and thiazole-2,4,5-tricarboxylic acid (TTCA) and thiazole-4,5-dicarboxylic acid (TDCA) from pheomelanin.
Quantification of these five markers by HPLC provides useful information on the quantity and structural diversity of melanins in various biological samples. HPLC analysis of these markers using the original method of 0.1 M potassium phosphate buffer (pH 2.1):methanol = 99:1 (85:15 for PTeCA) on a reversed-phase column had some problems, including the short lifetime of the column and, except for the major eumelanin marker PTCA, other markers were occasionally overlapped by interfering peaks in samples containing only trace levels of these markers.
These problems can be overcome by the addition of an ion pair reagent for anions, such as tetra-n-butylammonium bromide (1 mM), to retard the elution of di-, tri- and tetra-carboxylic acids. The methanol concentration was increased to 17% (30% for PTeCA) and the linearity, reproducibility, and recovery of the markers with this improved method is good to excellent.
This improved HPLC method was compared to the original method using synthetic melanins, mouse hair, human hair, and human epidermal samples. In addition to PTCA, TTCA, a major marker for pheomelanin, showed excellent correlations between both HPLC methods. The other markers showed an attenuation of the interfering peaks with the improved method. We recommend this improved HPLC method for the quantitative analysis of melanin markers following AHPO because of its simplicity, accuracy, and reproducibility.
Keywords: 4-amino-3-hydroxyphenylalanine (4-AHP); alkaline hydrogen peroxide oxidation (AHPO); high-performance liquid chromatography (HPLC); individual typology angle (ITA); pyrrole-2,3,4,5-tetracarboxylic acid (PTeCA); pyrrole-2,3,5-tricarboxylic acid (PTCA); pyrrole-2,3-dicarboxylic acid (PDCA); thiazole-2,4,5-tricarboxylic acid (TTCA); thiazole-4,5-dicarboxylic acid (TDCA); total melanin (TM).
Manipulating the click reactivity of dibenzoazacyclooctynes: from an azide clicker to a caged acylation reagent
Strain-promoted azide-alkyne cycloaddition using dibenzoazacyclooctyne (DBCO) is widely applied in copper-free bioorthogonal reactions. Here, we report the efficient acid-promoted rearrangement and silver-catalyzed amidation of DBCO, which alters its click reactivity robustly. In the switched click reaction, DBCO, as a caged acylation reagent, enables rapid peptide/protein modification after decaged by silver catalysts that renders site-specific conjugation of IgG antibody via an Fc-targeting peptide.
Keywords: DBCO; caged acylation reagent; click chemistry; silver catalyst; switched click reaction.
MOUSE SERUM:Azide Free Serum Products |
GWB-Q00010 |
GenWay Biotech |
5 ml |
Ask for price |
MOUSE SERUM:Azide Free Serum Products |
GWB-Q00011 |
GenWay Biotech |
10 ml |
Ask for price |
MOUSE SERUM:Azide Free Serum Products |
GWB-Q00012 |
GenWay Biotech |
50 ml |
Ask for price |
MOUSE SERUM:Azide Free Serum Products |
GWB-Q00013 |
GenWay Biotech |
100 ml |
Ask for price |
MOUSE SERUM:Azide Free Serum Products |
GWB-Q00014 |
GenWay Biotech |
500 ml |
Ask for price |
GUINEA PIG SERUM:Azide Free Serum Products |
GWB-Q00005 |
GenWay Biotech |
5 ml |
Ask for price |
PIG SERUM:Azide Free |
MBS239301-100mL |
MyBiosource |
100mL |
EUR 220 |
PIG SERUM:Azide Free |
MBS239301-5x100mL |
MyBiosource |
5x100mL |
EUR 805 |
Exo-Flow 2.0 Basic Kit without antibody (Streptavidin beads + reagents) - for Serum or Plasma |
EXOFLOW2-BASICA-SP |
SBI |
30 rxn |
EUR 1201.2 |
|
Bovine Serum Albumin Fatty Acid Free Reagent Grade |
GWB-F06E29 |
GenWay Biotech |
1 kg |
Ask for price |
Bovine Serum Albumin Fatty Acid Protease Free Reagent Grade |
GWB-DCC190 |
GenWay Biotech |
1 kg |
Ask for price |
RABBIT SERUM WITH 0.09% AZIDE Serum Products |
GWB-Q00017 |
GenWay Biotech |
20 ml |
Ask for price |
RABBIT SERUM WITH 0.09% AZIDE Serum Products |
GWB-Q00018 |
GenWay Biotech |
100 ml |
Ask for price |
Albumin, Human Serum (Azide Free) (HSA) |
MBS6120697-025mg |
MyBiosource |
0.25(mg |
EUR 495 |
Albumin, Human Serum (Azide Free) (HSA) |
MBS6120697-1mg |
MyBiosource |
1mg |
EUR 1000 |
Albumin, Human Serum (Azide Free) (HSA) |
MBS6120697-5x1mg |
MyBiosource |
5x1mg |
EUR 4350 |
VEX Exosome Isolation Reagent (from serum) |
R602 |
Vazyme |
10 ml |
EUR 769.2 |
Human Serum Albumin Antigen, Reagent Grade |
MBS568775-100g |
MyBiosource |
100g |
EUR 1240 |
Human Serum Albumin Antigen, Reagent Grade |
MBS568775-5x100g |
MyBiosource |
5x100g |
EUR 5385 |
EZ-DNA Reagents |
BS8202 |
Bio Basic |
100preps |
EUR 105.67 |
|
EZ-DNA Reagents |
SK8201 |
Bio Basic |
100preps |
EUR 112.2 |
|
ALB (ALB, Serum albumin) (Azide free) (HRP) |
MBS6121446-02mL |
MyBiosource |
0.2(mL |
EUR 980 |
ALB (ALB, Serum albumin) (Azide free) (HRP) |
MBS6121446-5x02mL |
MyBiosource |
5x0.2mL |
EUR 4250 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
7917-100 |
Biovision |
each |
EUR 783.6 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
7917-25 |
Biovision |
each |
EUR 294 |
Bovine Serum Albumin ? Heat Shock, Reagent Grade, pH 7.0 |
7917-5 |
Biovision |
each |
EUR 144 |
T-Pro Total Exosome Isolation reagent (from serum) |
JO66-V002M |
T-Pro Biotechnology |
25ml/BT |
EUR 800 |
T-Pro Total Exosome Isolation reagent (from serum) |
JO66-V002S |
T-Pro Biotechnology |
1ml*5/set |
EUR 200 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
MBS6120698-025mg |
MyBiosource |
0.25(mg |
EUR 445 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
MBS6120698-1mg |
MyBiosource |
1mg |
EUR 1015 |
Albumin, Human Serum (BSA) (BSA & Azide Free) |
MBS6120698-5x1mg |
MyBiosource |
5x1mg |
EUR 4415 |
Albumin Depletion Reagent for Plasma and Serum (10 ml) NEW! |
P5W13 |
101Bio |
10 rxn |
EUR 99 |
ExoQualiTM Overall Exosome Isolation Reagent (from serum) |
CEIK-044L-30T |
Creative Bioarray |
30 T |
Ask for price |
ExoQualiTM Overall Exosome Isolation Reagent (from serum) |
CEIK-044L-50T |
Creative Bioarray |
50 T |
Ask for price |
Albumin, Human Serum (BSA and Azide Free) (HSA) |
MBS6120696-1mg |
MyBiosource |
1(mg |
EUR 1055 |
Albumin, Human Serum (BSA and Azide Free) (HSA) |
MBS6120696-5x1mg |
MyBiosource |
5x1mg |
EUR 4600 |
OPEF01351-100G - Human Serum Albumin Antigen Reagent Grade |
OPEF01351-100G |
Aviva Systems Biology |
100g |
EUR 1302 |
|
MinuteTM Albumin Depletion Reagent for Plasma and Serum (20 ml) |
WA-013 |
Inventbiotech |
each |
EUR 135 |
CryoProtX Mix Reagents 1.5 mL |
M-MDSR-61 |
MiTeGen |
1.5 ml ml |
EUR 37 |
Description: CryoProtX Mix Reagents 1.5 mL |
Set of 10 Biolipidure Reagents |
Biolipidure-set |
AS ONE International |
10mLx10 |
EUR 1820.4 |
|
Description: Set of 10 Biolipidure Reagents, whose applications include Immunoassays, Western blots, Immunohistochemistry, Turbidimetric assays, Immunochromatography, and Bead based assays. Benefits include: No lot to lot variation, No animal derived materials, Non-specific adsorption suppression, Stabilization of immobilized antibody, Stabilization of enzyme-antibody conjugate, Enzyme-substrate reaction enhancement and aggregation reaction enhancement |
CryoProtX Mix Eco Reagents 1.5 mL |
M-MDSR-61-ECO |
MiTeGen |
1.5 ml ml |
EUR 37 |
Description: CryoProtX Mix Eco Reagents 1.5 mL |
Albumin Bovine (pH 7) fraction V Reagent Grade (Bovine Serum Albumin, BSA), 99% |
65186 |
Sisco Laboratories |
10 Gms |
EUR 38.4 |
|
Description: Part B |
PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP) |
MBS6491998-02mL |
MyBiosource |
0.2mL |
EUR 980 |
PON3, NT (Serum Paraoxonase/Lactonase 3) (Azide free) (HRP) |
MBS6491998-5x02mL |
MyBiosource |
5x0.2mL |
EUR 4250 |
PeliKine tool set, additional reagents |
M1980 |
Tecan Benelux |
1 unit |
EUR 169.8 |
Human IgG (serum origin, purified >97%, low endotoxin, azide free) |
20007-1-LE-1 |
Alpha Diagnostics |
1 mg |
EUR 196.8 |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
NSPF-1 |
Alpha Diagnostics |
1 ml |
EUR 343.2 |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
NSPF-10 |
Alpha Diagnostics |
10 ml |
EUR 781.2 |
Rabbit control serum (non-immunized, Disease free, SPF Rabbits) |
NSPF-5 |
Alpha Diagnostics |
5 ml |
EUR 634.8 |
SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP) |
MBS6344814-02mL |
MyBiosource |
0.2mL |
EUR 980 |
SAA4, CT (SAA4, CSAA, Serum amyloid A-4 protein, Constitutively expressed serum amyloid A protein) (Azide free) (HRP) |
MBS6344814-5x02mL |
MyBiosource |
5x0.2mL |
EUR 4250 |
Bradford(Coomassie) Protein Assay Plus Reagents |
P7201-050 |
GenDepot |
450ml |
EUR 225.6 |
Bradford(Coomassie) Protein Assay Plus Reagents |
P7201-100 |
GenDepot |
950ml |
EUR 130 |
PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
MBS6335888-02mL |
MyBiosource |
0.2mL |
EUR 980 |
PON2, ID (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
MBS6335888-5x02mL |
MyBiosource |
5x0.2mL |
EUR 4250 |
PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
MBS6335899-02mL |
MyBiosource |
0.2mL |
EUR 980 |
PON2, NT (PON2, Serum paraoxonase/arylesterase 2, Aromatic esterase 2, Serum aryldialkylphosphatase 2) (Azide free) (HRP) |
MBS6335899-5x02mL |
MyBiosource |
5x0.2mL |
EUR 4250 |
Pre-Titrated Reagents for Human EGF ELISA |
MBS590076-10Plates |
MyBiosource |
10Plates |
EUR 710 |
Pre-Titrated Reagents for Human EGF ELISA |
MBS590076-5Plates |
MyBiosource |
5Plates |
EUR 520 |
Pre-Titrated Reagents for Human EGF ELISA |
MBS590076-5x10Plates |
MyBiosource |
5x10Plates |
EUR 3270 |
RABBIT SERUM:Preservative Free |
MBS238207-20mL |
MyBiosource |
20mL |
EUR 190 |
RABBIT SERUM:Preservative Free |
MBS238207-5x20mL |
MyBiosource |
5x20mL |
EUR 675 |
RABBIT SERUM:Preservative Free |
MBS238208-100mL |
MyBiosource |
100mL |
EUR 230 |
RABBIT SERUM:Preservative Free |
MBS238208-5x100mL |
MyBiosource |
5x100mL |
EUR 800 |
CRP free serum |
MBS663102-1Unit |
MyBiosource |
1Unit |
EUR 875 |
CRP free serum |
MBS663102-5x1Unit |
MyBiosource |
5x1Unit |
EUR 3530 |
CRP Free Serum |
MBS318434-50mL |
MyBiosource |
50mL |
EUR 1115 |
CRP Free Serum |
MBS318434-5x50mL |
MyBiosource |
5x50mL |
EUR 4765 |
Liposomal and Non-Liposomal Transfection Reagents |
abx298032-100g |
Abbexa |
100 µg |
Ask for price |