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Asymmetric copper-catalyzed conjugate additions of organometallic reagents in the syntheses of natural compounds and pharmaceuticals.

Access to enantiopure complex molecular structures is crucial for the development of new drugs as well as agents used in crop-protection. In this regard, numerous asymmetric methods have been established. Copper-catalyzed 1,4-additions of organometallic reagents are robust C-C bond formation strategies applicable in a wide range of circumstances.
This review analyses the syntheses of natural products and pharmaceutical agents, which rely on the application of asymmetric Cu-catalyzed conjugate additions of various organometallic reagents. A wide range of available organometallics, e.g. dialkylzinc, trialkylaluminum, Grignard, and organozirconium, can now be used in conjugate additions to address various synthetic challenges present in targeted natural compounds. Furthermore, efficient catalysts allow high levels of stereofidelity over a diverse array of starting Michael acceptors.
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Taq DNA Polymerase

9001-500
EUR 229.2

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

L7051001 1000 unit
EUR 234
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 104.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 681.6
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 234
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq Plus DNA Polymerase

P201-01 250 U
EUR 147.6

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 213.6

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 382.8

Maximo Taq DNA Polymerase

S101 500 units
EUR 55.2

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 128.4

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 393.6

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 157.2

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 230.4

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 375.6

Recombinant Taq Dna Polymerase

27079804 EACH
EUR 99.18

Recombinant Taq Dna Polymerase

27079806 EACH
EUR 539.22

Taq DNA Polymerase Polyclonal Antibody

7018-100
EUR 424.8

Taq DNA Polymerase Polyclonal Antibody

7018-30T
EUR 175.2

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 620.4

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 399.6

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 286.8

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 710.4

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 276

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 393.6

Fast-Taq DNA Polymerase (500U)

9K-001-0036 500U
EUR 420.65

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U
EUR 260.4

ACTaq? Taq DNA Polymerase, 250U

E2100-250U
EUR 122.4

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 276

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 196.8

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 231.6

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 355.2
Description: Minimum order quantity: 1 unit of 500EU

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ100 100U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 476.4

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 176.4

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 273.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 314.4

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 314.4

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U
EUR 272.4

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U
EUR 126

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

Evaluation of a new thromboplastin reagent STA-NeoPTimal on a STA R Max analyzer for the measurement of prothrombin time, international normalized ratio and extrinsic factor levels

Introduction: We aimed at evaluating the performance of a new prothrombin time (PT) reagent (STA-NeoPTimal) with two other PT reagents (STA-Neoplastine R and STA-Neoplastine CI Plus) and the reference PT reagent used in our laboratory (ReadiPlasTin).
Methods: Evaluation consisted in intra- and interassay precision assessment, determination of sensitivity to unfractionated heparin (UFH) or enoxaparin in spiked samples and to direct oral anticoagulants (DOACs) in patients (n = 43). Method comparison of the 4 PT reagents, factor II, V, VII and X assays was tested on normal (n = 20) and abnormal samples: VKA (n = 47), preoperative (n = 23), liver failure (n = 12) and burned patients (n = 37).
Results: Analytical performance met manufacturers’ criteria for all reagents. All PT reagents gave correlation coefficients >0.8 and even >0.9 in many situations.
In some VKA samples, differences ≥ 0.5 INR units were found in samples within and above therapeutic ranges. For burned patients, PT correlations were good but with some minimal bias (<5.0%) while factor assays gave very consistent results (R > .8 and mainly >0.9). As expected, poor responsiveness of the PT to DOAC concentrations was observed with all four assays.
Conclusion: The STA-NeoPTimal showed comparable performance to ReadiPlasTin, making it suitable for VKA control, detection of factors II, V, VII, X deficiency and assessment of liver disease coagulopathy.
However, for patients receiving VKA, some significant differences were observed. We confirmed the inability of the PT assay to detect residual DOAC concentrations. Finally, burned patients results showed that recombinant thromboplastins were less sensitive to factor deficiencies in comparison to extraction thromboplastins.

An Experimental and Theoretical Study on the “2,2′ Bipiridyl Ni Catalyzed” Hirao Reaction of >P(O)H Reagents and Halobenzenes; A Ni(0)→Ni(II) or a Ni(II)→Ni(IV) mechanism?

The P-C couplings between >P(O)H reagents and halogenoarenes may take place either by Pd- or Ni-catalysis. However, the latter version has been less studied. It was found by us that the reaction of Ph2P(O)H or PhEtOP(O)H with PhX (X = I and Br) in the presence of NiCl2/Zn as the precursors for the assumed Ni(0) complexant together with 2,2′-bipyridine as the ligand took place only with PhI at 50/70 °C. M06-2X/6-31G(d,p)//PCM(MeCN) calculations for the P-C coupling of Ph2P(O)H and PhX revealed a favorable energetics only for the loss of iodide following the oxidative addition of PhI on the Ni(0) atom.
  • However, the assumed transition states with Ni(II) formed after P-ligand uptake and deprotonation could not undergo reductive elimination meaning a “dead-end street” in the mechanism expected. Hence, it was assumed that the initial complexation of the remaining Ni2+ ions with 2,2′-bipyridine may move the P-C coupling forward via a Ni(II)→Ni(IV) transition.
  • This route was also evaluated by calculations, and the mechanism explored was justified by preparative experiments carried out using NiCl2 and bipyridine, but in the absence of Zn. There was no need for Zn, when bipyridine was applied as the N-ligand, moreover PhBr could also be a suitable starting material at higher temperatures.
  • Hence, the generally accepted Ni(0)→Ni(II) route was refuted by us, confirming, surprisingly, the generality of the Ni(II)→N(IV) protocol, either in the presence of bipyridine, or using the excess of the >P(O)H ⇌ >P-OH reagent as the P-ligand.
  • The results of the calculations on the complex forming ability of Ni(0) and Ni(II) with 2,2′-bipyridine or the tautomeric >P-OH form of the P-reagents were in accord with our mechanistic proposition. Our study also means the clarification of the contradicting and sometimes uncertain literature data published in the last decade.

10 mM dNTP Mix of 2.5 mM 4 dNTP Mix

GR103045 1 mL
EUR 75.6

dNTP MIX 100mM, 1ml

2013MIX-1ML
EUR 184.8

dNTP Mix, 25mM each

40053 1mL
EUR 312
Description: Minimum order quantity: 1 unit of 1mL

dNTP Mix, 10mM each

40054 5x1mL
EUR 550.8
Description: Minimum order quantity: 1 unit of 5x1mL

dNTP Mix (1 ml)

9002-1
EUR 183.6

High Quality dNTP Mix, 10mM

ABP-PP-ERDN100 1mL Ask for price

High Quality dNTP Mix, 10mM

ABP-PP-ERDN500 5mL Ask for price

dNTP Mix, 100mM Final Conc.

BIO-39028 50µmol, (1 x 500µl) Ask for price

dNTP Mix, 100mM Final Conc.

BIO-39029 200µmol, (4 x 500µl) Ask for price

dNTP Mix, 40mM Final Conc.

BIO-39043 20µmol, (1 x 500µl) Ask for price

dNTP Mix, 10mM Final Conc.

BIO-39044 10µmol, (1ml vol.) Ask for price

dNTP Mix, 10mM Final Conc.

BIO-39053 100µmol, (10 x 1ml) Ask for price

dNTP Mix (10 mM each)

P031-01 1 ml
EUR 142.8

dNTP Mix (10 mM each)

P031-02 5 ml
EUR 213.6

dNTP Mix (2.5 mM each)

P032-01 1 ml
EUR 129.6

dNTP Mix (2.5 mM each)

P032-02 5 ml
EUR 162

Volatile Organics Mix

60-BIG-MIX 1ML
EUR 120.84

5 Component Mix

AOAC-MIX-1 EACH
EUR 117.42

dNTP Mix (10 mM each) 1ml

9K-003-0006 1ml
EUR 174.84

dNTP Mix (10 mM each) 5x1ml

9K-003-0013 5x1mL
EUR 615.06

40mM dNTP Mix (10mM/20µmol each)

PR3040-M-1 1 PC
EUR 118.2

40mM dNTP Mix (10mM/80µm each)

PR3040-M-4 1 PC
EUR 190.93

ReadiUse™ dNTP Mix *10 mM*

17200 5 mL
EUR 318

Semi-volatile Organics Mix

76-BIG-MIX 1ML
EUR 150.48

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1536.74

Fast-Pfu with 10mM dNTP Mix (250U)

9K-001-0022 250U
EUR 482.82

Fast-Pfu with 10mM dNTP Mix (500U)

9K-001-0023 500U
EUR 741.22

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 351.28

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 518.84

Volatile Organics Mix (76 Components)

8260-BIG-MIX 1ML
EUR 296.4

LR DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0008 250U
EUR 404.52

Pfu DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0009 500U
EUR 686.4

Pfu DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0010 250U
EUR 396.7

Volatile Organics Mix (Low Level)

60-BIG-MIX-200 1ML
EUR 116.28

Volatile Organics Mix (High Level)

60-BIG-MIX-2000 1ML
EUR 165.3

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 802.8

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 575.22

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2465.38

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 384.17

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

9K-001-0006 4x250U
EUR 1055.98

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

9K-001-0020 5000U
EUR 2878.8

Pfu DNA Polymerase with 10mM dNTP Mix (10, 000U)

9K-001-0021 10000U
EUR 5384.4

ReadiUse™ dNTP Mix Set *10 mM PCR Grade*

17258 1 mL
EUR 79

dNTP Set

G050 4 x 250 ul (100 mM each)
EUR 133.2

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 428.4

dNTP Set, 100mM

BIO-39025 4 x 25µmol, (4 x 250µl) Ask for price

dNTP Set, 100mM

BIO-39026 4 x 100µmol, (4 x 4 x 250µl) Ask for price

dNTP Set, 100mM

BIO-39049 4 x 100µmol, (4 x 1ml) Ask for price

dNTP mixture (10mM)

DD0056 0.5ml
EUR 75.66

dNTP mixture (25mM)

DD0057 0.5ml
EUR 95.5

dNTP 10 mM

FYT013-200UL 200 µl Ask for price

100mM dNTP Set

PR3101-S-1 1 PC
EUR 204.32

100mM dNTP Set

PR3101-S-4 1 PC
EUR 516.31

dNTP Set, 100mM each

40052 4x250uL
EUR 312
Description: Minimum order quantity: 1 unit of 4x250uL

dNTP Set (100mM each) - 4x0.5ml

DD0058 4x0.5ml
EUR 247.92

HiFi DNA Polymerase wi dNTP

FYT003-500U 500U Ask for price

Mix 524.3 Mix B 69 Components

5243-VCM 1ML
EUR 404.7

PCR Mix

L5051100 2.5 ml
EUR 128.4
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

NTP Mix

G943 250 ul 25 mM each
EUR 93.6

NTP Mix

G944 500 ul 25 mM each
EUR 116.4

NTP Mix

G945 1.0 ml 25 mM each
EUR 156

Benzidines Mix

605-X-MC 1ML
EUR 42.18

Analyte Mix

608-A 1ML
EUR 67.26

Alcohols Mix

8015B-A 1ML
EUR 44.46

Analyte Mix

8140-A 1ML
EUR 165.3

Analyte Mix

8141-AB 1ML
EUR 182.4

Analyte Mix

5031-A 1ML
EUR 166.44

Analyte Mix

5491-A 1ML
EUR 45.6

Phenolics Mix

CLPS-A 1ML
EUR 46.74

Aliphatics Fractionation Check Mix (18 Component Mix)

NJDEP-EPH-ALFC 5ML
EUR 229.14

Aliphatics Matrix Spike Mix (18 Component Mix)

NJDEP-EPH-ALMS 5ML
EUR 212.04

Aromatics Fraction Check Mix (16 Component Mix)

NJDEP-EPH-ARFS 5ML
EUR 239.4

Aromatics Matrix Spike Mix (18 Component Mix)

NJDEP-EPH-ARMS 5ML
EUR 221.16

dNTP Set 100 mM 4x0.4 ml

BH40203 4x0.4ml
EUR 178.8
Description: High purity buffer for various PCR applications.

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

Taq Plus DNA Polmerase (with dNTP)

P201-d1 250 U
EUR 151.2

Taq Plus DNA Polmerase (with dNTP)

P201-d2 1000 U
EUR 231.6

Taq Plus DNA Polmerase (with dNTP)

P201-d3 3000 U
EUR 424.8

Glucose Nutrient Mix

109 150 g
EUR 80.4

ACCUZYME Mix, 2x

BIO-25028 500 Reactions Ask for price

MyTaq Mix, 2x

BIO-25041 200 Reactions Ask for price

MyTaq Mix, 2x

BIO-25041/S Sample Ask for price

MyTaq Mix, 2x

BIO-25042 1000 Reactions Ask for price

MyFi Mix, 2x

BIO-25049 100 Reactions Ask for price

MyFi Mix, 2x

BIO-25049/S Sample Ask for price