naclai

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Asymmetric copper-catalyzed conjugate additions of organometallic reagents in the syntheses of natural compounds and pharmaceuticals.

Access to enantiopure complex molecular structures is crucial for the development of new drugs as well as agents used in crop-protection. In this regard, numerous asymmetric methods have been established. Copper-catalyzed 1,4-additions of organometallic reagents are robust C-C bond formation strategies applicable in a wide range of circumstances.
This review analyses the syntheses of natural products and pharmaceutical agents, which rely on the application of asymmetric Cu-catalyzed conjugate additions of various organometallic reagents. A wide range of available organometallics, e.g. dialkylzinc, trialkylaluminum, Grignard, and organozirconium, can now be used in conjugate additions to address various synthetic challenges present in targeted natural compounds. Furthermore, efficient catalysts allow high levels of stereofidelity over a diverse array of starting Michael acceptors.
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Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00103 500U
EUR 104.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

L7051001 1000 unit
EUR 167

Taq DNA Polymerase

L7051200 200 unit
EUR 43

Taq DNA Polymerase

MB101-0500 500 U/100 μl
EUR 10

Taq DNA Polymerase

abx299725-100g 100 µg Ask for price

Taq DNA Polymerase

abx299725-20g 20 µg
EUR 337.5

Taq DNA Polymerase

abx299725-50g 50 µg Ask for price

Taq DNA Polymerase

abx461029-1096tests 10 × 96 tests
EUR 1787.5

Taq DNA Polymerase

abx461029-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase

abx461029-96tests 96 tests
EUR 362.5

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

TM Taq DNA Polymerase

TBS4010 1000 U
EUR 60

Taq HS DNA Polymerase

P132-d1 500 U
EUR 47.85

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 89.18

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 406

Taq DNA Polymerase Mix

abx461043-1096tests 10 × 96 tests Ask for price

Taq DNA Polymerase Mix

abx461043-596tests 5 × 96 tests
EUR 1062.5

Taq DNA Polymerase Mix

abx461043-96tests 96 tests
EUR 337.5

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 157.2

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 230.4

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 375.6

Maximo Taq DNA Polymerase

S101 500 units
EUR 55.2

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 128.4

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 393.6

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 595

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 167

Taq DNA Polymerase -1000 units

3243 1000units
EUR 115
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 449
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Recombinant Taq Dna Polymerase

27079806 EACH
EUR 607.62

Taq DNA Polymerase (Recombinant)

22060707-1 1,000 Unit(s)
EUR 137.27

Taq DNA Polymerase (Recombinant)

22060707-2 1500 Unit(s)
EUR 182.1

Taq DNA Polymerase (Recombinant)

22060707-3 5000 Unit(s)
EUR 465.77

Taq DNA Polymerase (Recombinant)

22060707-4 10 KU
EUR 840.96

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Taq DNA Polymerase Recombinant

rAP-1062 Inquiry Ask for price

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 231.6

ACTaq? Taq DNA Polymerase, 250U

E2100-250U each
EUR 122.4

TaqFast DNA Polymerase (PCR)

G277 250 U (50 ul)
EUR 87.6

TaqFast DNA Polymerase (PCR)

G278 1000 U (200 ul)
EUR 160.8

Taq Plus DNA Polymerase

P201-01 250 U
EUR 147.6

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 213.6

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 382.8

Taq DNA Polymerase (5U/uL)

A1003-500U 500U
EUR 26.4
Description: Biotechnology

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

Taq Plus DNA Polymerase (Taq Plus DNA) Protein

abx073522-100tests 100 tests
EUR 225

Taq DNA Polymerase (5U/µl), 2000U

PL1204 each Ask for price

Hot start Taq DNA Polymerase

EQ006 100μl/500U
EUR 200

Hot Start Taq DNA Polymerase

abx460010-1096tests 10 × 96 tests Ask for price

Hot Start Taq DNA Polymerase

abx460010-596tests 5 × 96 tests Ask for price

Hot Start Taq DNA Polymerase

abx460010-96tests 96 tests
EUR 262.5

Taq DNA Polymerase (5U/µl), 500U

PL1202 each Ask for price

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Taq DNA Polymerase (TaqDNA) Protein

abx073521-100tests 100 tests
EUR 225

Apex Red Taq DNA Polymerase, 10000u

42-402R 20 x 500u/Unit
EUR 1229.49
Description: 10X NH4 Buffer, Mg Free

Evaluation of a new thromboplastin reagent STA-NeoPTimal on a STA R Max analyzer for the measurement of prothrombin time, international normalized ratio and extrinsic factor levels

Introduction: We aimed at evaluating the performance of a new prothrombin time (PT) reagent (STA-NeoPTimal) with two other PT reagents (STA-Neoplastine R and STA-Neoplastine CI Plus) and the reference PT reagent used in our laboratory (ReadiPlasTin).
Methods: Evaluation consisted in intra- and interassay precision assessment, determination of sensitivity to unfractionated heparin (UFH) or enoxaparin in spiked samples and to direct oral anticoagulants (DOACs) in patients (n = 43). Method comparison of the 4 PT reagents, factor II, V, VII and X assays was tested on normal (n = 20) and abnormal samples: VKA (n = 47), preoperative (n = 23), liver failure (n = 12) and burned patients (n = 37).
Results: Analytical performance met manufacturers’ criteria for all reagents. All PT reagents gave correlation coefficients >0.8 and even >0.9 in many situations.
In some VKA samples, differences ≥ 0.5 INR units were found in samples within and above therapeutic ranges. For burned patients, PT correlations were good but with some minimal bias (<5.0%) while factor assays gave very consistent results (R > .8 and mainly >0.9). As expected, poor responsiveness of the PT to DOAC concentrations was observed with all four assays.
Conclusion: The STA-NeoPTimal showed comparable performance to ReadiPlasTin, making it suitable for VKA control, detection of factors II, V, VII, X deficiency and assessment of liver disease coagulopathy.
However, for patients receiving VKA, some significant differences were observed. We confirmed the inability of the PT assay to detect residual DOAC concentrations. Finally, burned patients results showed that recombinant thromboplastins were less sensitive to factor deficiencies in comparison to extraction thromboplastins.

An Experimental and Theoretical Study on the “2,2′ Bipiridyl Ni Catalyzed” Hirao Reaction of >P(O)H Reagents and Halobenzenes; A Ni(0)→Ni(II) or a Ni(II)→Ni(IV) mechanism?

The P-C couplings between >P(O)H reagents and halogenoarenes may take place either by Pd- or Ni-catalysis. However, the latter version has been less studied. It was found by us that the reaction of Ph2P(O)H or PhEtOP(O)H with PhX (X = I and Br) in the presence of NiCl2/Zn as the precursors for the assumed Ni(0) complexant together with 2,2′-bipyridine as the ligand took place only with PhI at 50/70 °C. M06-2X/6-31G(d,p)//PCM(MeCN) calculations for the P-C coupling of Ph2P(O)H and PhX revealed a favorable energetics only for the loss of iodide following the oxidative addition of PhI on the Ni(0) atom.
  • However, the assumed transition states with Ni(II) formed after P-ligand uptake and deprotonation could not undergo reductive elimination meaning a “dead-end street” in the mechanism expected. Hence, it was assumed that the initial complexation of the remaining Ni2+ ions with 2,2′-bipyridine may move the P-C coupling forward via a Ni(II)→Ni(IV) transition.
  • This route was also evaluated by calculations, and the mechanism explored was justified by preparative experiments carried out using NiCl2 and bipyridine, but in the absence of Zn. There was no need for Zn, when bipyridine was applied as the N-ligand, moreover PhBr could also be a suitable starting material at higher temperatures.
  • Hence, the generally accepted Ni(0)→Ni(II) route was refuted by us, confirming, surprisingly, the generality of the Ni(II)→N(IV) protocol, either in the presence of bipyridine, or using the excess of the >P(O)H ⇌ >P-OH reagent as the P-ligand.
  • The results of the calculations on the complex forming ability of Ni(0) and Ni(II) with 2,2′-bipyridine or the tautomeric >P-OH form of the P-reagents were in accord with our mechanistic proposition. Our study also means the clarification of the contradicting and sometimes uncertain literature data published in the last decade.

dNTP Mix

K6011105-200 200 ul (10 mM each)
EUR 53

dNTP Mix

K6011105-400 400 ul (10 mM each)
EUR 81

10 mM dNTP Mix of 2.5 mM 4 dNTP Mix

GR103045 1 mL
EUR 63

dNTP Mix (100 mM)

abx461012-1096tests 10 × 96 tests Ask for price

dNTP Mix (100 mM)

abx461012-596tests 5 × 96 tests
EUR 850

dNTP Mix (100 mM)

abx461012-96tests 96 tests
EUR 337.5

dNTP MIX 100mM, 1ml

2013MIX 1ML
EUR 90.35

dNTP MIX 100mM, 1ml

2013MIX-1ML each
EUR 184.8

dNTP/dUTP Mix

DB2236 1ml
EUR 70.44

dNTP Mix (1 ml)

9002-1 each
EUR 183.6

10 mM dNTP Mix

DN0010 1 ml
EUR 14

10 mM dNTP Mix

abx299724-100g 100 µg Ask for price

10 mM dNTP Mix

abx299724-20g 20 µg
EUR 287.5

10 mM dNTP Mix

abx299724-50g 50 µg Ask for price

dNTP Mix (10 mM)

abx461013-1096tests 10 × 96 tests Ask for price

dNTP Mix (10 mM)

abx461013-596tests 5 × 96 tests
EUR 637.5

dNTP Mix (10 mM)

abx461013-96tests 96 tests
EUR 250

dNTP Mix (40 mM)

abx461014-1096tests 10 × 96 tests Ask for price

dNTP Mix (40 mM)

abx461014-596tests 5 × 96 tests Ask for price

dNTP Mix (40 mM)

abx461014-96tests 96 tests
EUR 250

2mM dNTP Mix, 1ml

NP2411 each Ask for price

10mM dNTP Mix, 1ml

NP2410 each Ask for price

dNTP Mix, 25mM each

40053-1 EA
EUR 237

dNTP Mix, 10mM Each

A2446-05ML 0.5ML
EUR 16
Description: Biotechnology

dNTP Mix, 25mM Each

A2447-05ML 0.5ML
EUR 40
Description: Biotechnology

10mM dNTP Mix, 0.25ml

NP2409 each Ask for price

25mM dNTP Mix, 0.25ml

NP2413 each Ask for price

dNTP Mix dUTP - Solution

NU-1020L 5x 1ml
EUR 309.2

dNTP Mix dUTP - Solution

NU-1020S 1ml
EUR 77.3

dNTP Mix, 100mM Final Conc.

BIO-39028 50µmol, (1 x 500µl) Ask for price

dNTP Mix, 100mM Final Conc.

BIO-39029 200µmol, (4 x 500µl) Ask for price

dNTP Mix (10 mM each)

P031-01 1 ml
EUR 24.8

dNTP Mix (10 mM each)

P031-02 5 ml
EUR 108.5

dNTP Mix (2.5 mM each)

P032-01 1 ml
EUR 129.6

dNTP Mix (2.5 mM each)

P032-02 5 ml
EUR 162

Apex dNTP Mix, 2 x 100µmol

42-405 2 x 1ml/Unit
EUR 390.89
Description: 25mM each, 100mM Total

dNTP Mix - 10 mM Solution

NU-1006-10ML 10ml
EUR 299.9

dNTP Mix - 10 mM Solution

NU-1006L 5x 1ml
EUR 257.7

dNTP Mix - 10 mM Solution

NU-1006S 1ml
EUR 64.3

dNTP Mix - 25 mM Solution

NU-1023-10ML 10ml
EUR 641.4

dNTP Mix - 25 mM Solution

NU-1023L 5x 1ml
EUR 441.8

dNTP Mix - 25 mM Solution

NU-1023S 1ml
EUR 110.3

ReadiUseâ„¢ dNTP Mix *10 mM*

17200 5 mL
EUR 325
Description: Our Ready-to Use Solution of deoxynucleotide triphosphates (dNTPs) have been extensively tested and verified for use in a wide variety of molecular biology applications, including PCR, real-time PCR, high-fidelity PCR, long PCR, cDNA synthesis, RT-PCR, RCA, MDA, DNA labeling, DNA sequencing, in vitro transcription, siRNA synthesis, and RNA amplification.

ReadiUseâ„¢ dNTP Mix *10 mM*

17200-5mL 5 mL
EUR 318
Description: Our Ready-to Use Solution of deoxynucleotide triphosphates (dNTPs) have been extensively tested and verified for use in a wide variety of molecular biology applications, including PCR, real-time PCR, high-fidelity PCR, long PCR, cDNA synthesis, RT-PCR, RCA, MDA, DNA labeling, DNA sequencing, in vitro transcription, siRNA synthesis, and RNA amplification.

dNTP Mix, 10mM each, 5x1mL

40054-1 PK
EUR 460

dNTP Mix, 40mM Final Conc.

BIO-39043 20µmol, (1 x 500µl) Ask for price

dNTP Mix, 10mM Final Conc.

BIO-39044 10µmol, (1ml vol.) Ask for price

dNTP Mix, 10mM Final Conc.

BIO-39053 100µmol, (10 x 1ml) Ask for price

Apex dNTP Mix, 10 x 10µmol

42-406 10 x 1ml/Unit
EUR 303.81
Description: 2.5mM each, 10mM Total

Apex dNTP Mix, 5 x 20µmol

42-407 5 x 500µl/Unit
EUR 877.07
Description: 10mM each, 40mM Total

Apex dNTP Mix, 1 x 20µmol

42-411 1 x 500µl/Unit
EUR 129.2
Description: 10mM each, 40mM Total

High Quality dNTP Mix, 10mM

ABP-PP-ERDN100 1mL Ask for price

High Quality dNTP Mix, 10mM

ABP-PP-ERDN500 5mL Ask for price

TM dNTP Mix, 10 mM/each

TBS4020 0.5 ml
EUR 38

2mM dNTP Mix, 5 x 1ml

NP2412 each Ask for price

dNTP Deoxynucleotide Solution Mix

IDNTPDSM1ML each
EUR 70
Description: dNTP Deoxynucleotide Solution Mix

dNTP Mix (10 mM each) 1ml

9K-003-0006 1ml
EUR 174.84

25mM dNTP Mix, 5 x 0.25ml

NP2414 each Ask for price

dNTP Mix (10 mM each) 5x1ml

9K-003-0013 5x1mL
EUR 615.06

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1536.74

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 518.84

dNTP Mix, 40mM (A ready to use mix of dA

MBT187-0.2ML 1 unit
EUR 14.52
Description: dNTP Mix, 40mM (A ready to use mix of dA

dNTP Mix, 40mM (A ready to use mix of dA

MBT187-1ML 1 unit
EUR 43.21
Description: dNTP Mix, 40mM (A ready to use mix of dA

Fast-Pfu with 10mM dNTP Mix (250U)

9K-001-0022 250U
EUR 482.82

Fast-Pfu with 10mM dNTP Mix (500U)

9K-001-0023 500U
EUR 741.22

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 351.28

dNTP Mix(PCR Grade)10 mM each

EQ011 100μl
EUR 12

40mM dNTP Mix (10mM/80µm each), 2ml

PR3040-M-4 1 PC
EUR 157.59

40mM dNTP Mix (10mM/20µmol each), 0.5ml

PR3040-M-1 1 PC
EUR 42.44

LR DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0008 250U
EUR 404.52

Pfu DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0009 500U
EUR 686.4

Pfu DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0010 250U
EUR 396.7

Taq DNA Polymerase with dNTP Mix Buffer

11140015-1 500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-2 2500 Unit (s) Ask for price

Taq DNA Polymerase with dNTP Mix Buffer

11140015-3 5000 Unit (s) Ask for price

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2465.38

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

9K-001-0020 5000U
EUR 2878.8

Pfu DNA Polymerase with 10mM dNTP Mix (10, 000U)

9K-001-0021 10000U
EUR 5384.4

ReadiUseâ„¢ dNTP Mix Set *10 mM PCR Grade*

17258 1 mL
EUR 81
Description: 10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water.

ReadiUseâ„¢ dNTP Mix Set *10 mM PCR Grade*

17258-1mL 1 mL
EUR 79
Description: 10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water.

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 575.22

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 384.17

Taq DNA Polymerase - 1000 units with separate dNTP Mix

3243d 1000units
EUR 150
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 595
Description: Intact Genomics Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). This product is supplied with 10x PCR reaction buffer, containing MgCl2, which produces a final Mg2+ concentration of 1.5 mM. Ideal for primary extension reaction with DNA fragments having dA overhang on 3’ ends.Product Includes:Taq DNA Polymerase10x PCR Buffer with Mg2+5x Magic Enhancer10mM dNTP Mix

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

9K-001-0006 4x250U
EUR 1055.98