naclai

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Asymmetric copper-catalyzed conjugate additions of organometallic reagents in the syntheses of natural compounds and pharmaceuticals.

Access to enantiopure complex molecular structures is crucial for the development of new drugs as well as agents used in crop-protection. In this regard, numerous asymmetric methods have been established. Copper-catalyzed 1,4-additions of organometallic reagents are robust C-C bond formation strategies applicable in a wide range of circumstances.
This review analyses the syntheses of natural products and pharmaceutical agents, which rely on the application of asymmetric Cu-catalyzed conjugate additions of various organometallic reagents. A wide range of available organometallics, e.g. dialkylzinc, trialkylaluminum, Grignard, and organozirconium, can now be used in conjugate additions to address various synthetic challenges present in targeted natural compounds. Furthermore, efficient catalysts allow high levels of stereofidelity over a diverse array of starting Michael acceptors.
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Taq DNA Polymerase

BA00104 1000U
EUR 123
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 232
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 80
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 109
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 167
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

L7051001 1000 unit
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

L7051200 200 unit
EUR 87
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Taq DNA Polymerase

9001-2500
EUR 512

Taq DNA Polymerase

9001-500
EUR 191

Maximo Taq DNA Polymerase

S101 500 units
EUR 46

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 107

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 328

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 153
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 257
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 140
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 218
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 174.41

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 485

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1028.75

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 348.85

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 592

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 230

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 328

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 131

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 192

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 313

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 568
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 195
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 177

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 177

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 177

Taq Plus DNA Polymerase

P201-01 250 U
EUR 123

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 178

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 319

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U
EUR 217

ACTaq? Taq DNA Polymerase, 250U

E2100-250U
EUR 102

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Fast-Taq DNA Polymerase (500U)

9K-001-0036 500U
EUR 350.54

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 93.5

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 592

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 230

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 328

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 517

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Anti-Taq DNA Polymerase Antibody

48369-100ul 100ul
EUR 333

Anti-Taq DNA Polymerase Antibody

48369-50ul 50ul
EUR 239

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 164

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

Taq DNA Polymerase Polyclonal Antibody

7018-100
EUR 354

Taq DNA Polymerase Polyclonal Antibody

7018-30T
EUR 146

Taq DNA polymerase (TQ21) , DNA Aptamer, Biotinylated

AD-146-B Custom Ask for price

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 72

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 193

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 79

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U
EUR 227

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U
EUR 105

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

amfiSure Hot-Start Taq DNA Polymerase

P0322-010 100 units
EUR 147

amfiSure Hot-Start Taq DNA Polymerase

P0322-025 250 units
EUR 228

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 296
Description: Minimum order quantity: 1 unit of 500EU

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B01 10,000 units
EUR 1157

Taq DNA Polymerase, 5units/㎕ , Bulk Packing

T2200-B05 5x10,000 units
EUR 5450

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-050 500units
EUR 131

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-100 2x500 units
EUR 192

HiQTM Taq Red DNA Polymerase, 1unit/㎕

T2201-200 4x500 units
EUR 313

Recombinant (E.Coli) Taq Plus DNA Polymerase

RP-395 250 U
EUR 164

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ100 100U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price

Anti-Taq DNA Polymerase Conjugated Antibody

C48369 100ul
EUR 397

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 121

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 193

Evaluation of a new thromboplastin reagent STA-NeoPTimal on a STA R Max analyzer for the measurement of prothrombin time, international normalized ratio and extrinsic factor levels

Introduction: We aimed at evaluating the performance of a new prothrombin time (PT) reagent (STA-NeoPTimal) with two other PT reagents (STA-Neoplastine R and STA-Neoplastine CI Plus) and the reference PT reagent used in our laboratory (ReadiPlasTin).
Methods: Evaluation consisted in intra- and interassay precision assessment, determination of sensitivity to unfractionated heparin (UFH) or enoxaparin in spiked samples and to direct oral anticoagulants (DOACs) in patients (n = 43). Method comparison of the 4 PT reagents, factor II, V, VII and X assays was tested on normal (n = 20) and abnormal samples: VKA (n = 47), preoperative (n = 23), liver failure (n = 12) and burned patients (n = 37).
Results: Analytical performance met manufacturers’ criteria for all reagents. All PT reagents gave correlation coefficients >0.8 and even >0.9 in many situations.
In some VKA samples, differences ≥ 0.5 INR units were found in samples within and above therapeutic ranges. For burned patients, PT correlations were good but with some minimal bias (<5.0%) while factor assays gave very consistent results (R > .8 and mainly >0.9). As expected, poor responsiveness of the PT to DOAC concentrations was observed with all four assays.
Conclusion: The STA-NeoPTimal showed comparable performance to ReadiPlasTin, making it suitable for VKA control, detection of factors II, V, VII, X deficiency and assessment of liver disease coagulopathy.
However, for patients receiving VKA, some significant differences were observed. We confirmed the inability of the PT assay to detect residual DOAC concentrations. Finally, burned patients results showed that recombinant thromboplastins were less sensitive to factor deficiencies in comparison to extraction thromboplastins.

An Experimental and Theoretical Study on the “2,2′ Bipiridyl Ni Catalyzed” Hirao Reaction of >P(O)H Reagents and Halobenzenes; A Ni(0)→Ni(II) or a Ni(II)→Ni(IV) mechanism?

The P-C couplings between >P(O)H reagents and halogenoarenes may take place either by Pd- or Ni-catalysis. However, the latter version has been less studied. It was found by us that the reaction of Ph2P(O)H or PhEtOP(O)H with PhX (X = I and Br) in the presence of NiCl2/Zn as the precursors for the assumed Ni(0) complexant together with 2,2′-bipyridine as the ligand took place only with PhI at 50/70 °C. M06-2X/6-31G(d,p)//PCM(MeCN) calculations for the P-C coupling of Ph2P(O)H and PhX revealed a favorable energetics only for the loss of iodide following the oxidative addition of PhI on the Ni(0) atom.
  • However, the assumed transition states with Ni(II) formed after P-ligand uptake and deprotonation could not undergo reductive elimination meaning a “dead-end street” in the mechanism expected. Hence, it was assumed that the initial complexation of the remaining Ni2+ ions with 2,2′-bipyridine may move the P-C coupling forward via a Ni(II)→Ni(IV) transition.
  • This route was also evaluated by calculations, and the mechanism explored was justified by preparative experiments carried out using NiCl2 and bipyridine, but in the absence of Zn. There was no need for Zn, when bipyridine was applied as the N-ligand, moreover PhBr could also be a suitable starting material at higher temperatures.
  • Hence, the generally accepted Ni(0)→Ni(II) route was refuted by us, confirming, surprisingly, the generality of the Ni(II)→N(IV) protocol, either in the presence of bipyridine, or using the excess of the >P(O)H ⇌ >P-OH reagent as the P-ligand.
  • The results of the calculations on the complex forming ability of Ni(0) and Ni(II) with 2,2′-bipyridine or the tautomeric >P-OH form of the P-reagents were in accord with our mechanistic proposition. Our study also means the clarification of the contradicting and sometimes uncertain literature data published in the last decade.

10 mM dNTP Mix of 2.5 mM 4 dNTP Mix

GR103045 1 mL
EUR 63

dNTP MIX 100mM, 1ml

2013MIX-1ML
EUR 154

dNTP Mix, 25mM each

40053 1mL
EUR 260
Description: Minimum order quantity: 1 unit of 1mL

dNTP Mix, 10mM each

40054 5x1mL
EUR 459
Description: Minimum order quantity: 1 unit of 5x1mL

dNTP Mix (1 ml)

9002-1
EUR 153

dNTP Mix, 100mM Final Conc.

BIO-39028 50µmol, (1 x 500µl) Ask for price

dNTP Mix, 100mM Final Conc.

BIO-39029 200µmol, (4 x 500µl) Ask for price

dNTP Mix, 40mM Final Conc.

BIO-39043 20µmol, (1 x 500µl) Ask for price

dNTP Mix, 10mM Final Conc.

BIO-39044 10µmol, (1ml vol.) Ask for price

dNTP Mix, 10mM Final Conc.

BIO-39053 100µmol, (10 x 1ml) Ask for price

High Quality dNTP Mix, 10mM

ABP-PP-ERDN100 1mL Ask for price

High Quality dNTP Mix, 10mM

ABP-PP-ERDN500 5mL Ask for price

dNTP Mix (10 mM each)

P031-01 1 ml
EUR 119

dNTP Mix (10 mM each)

P031-02 5 ml
EUR 178

dNTP Mix (2.5 mM each)

P032-01 1 ml
EUR 108

dNTP Mix (2.5 mM each)

P032-02 5 ml
EUR 135

dNTP Mix (10 mM each) 1ml

9K-003-0006 1ml
EUR 145.7

dNTP Mix (10 mM each) 5x1ml

9K-003-0013 5x1mL
EUR 512.55

40mM dNTP Mix (10mM/20µmol each)

PR3040-M-1 1 PC
EUR 98.5

40mM dNTP Mix (10mM/80µm each)

PR3040-M-4 1 PC
EUR 159.11

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1280.62

Fast-Pfu with 10mM dNTP Mix (250U)

9K-001-0022 250U
EUR 402.35

Fast-Pfu with 10mM dNTP Mix (500U)

9K-001-0023 500U
EUR 617.68

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 292.73

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 907.39

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 432.37

LR DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0008 250U
EUR 337.1

Pfu DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0009 500U
EUR 572

Pfu DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0010 250U
EUR 330.58

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 479.35

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2054.48

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 320.14

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

9K-001-0006 4x250U
EUR 879.98

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

9K-001-0020 5000U
EUR 2399

Pfu DNA Polymerase with 10mM dNTP Mix (10, 000U)

9K-001-0021 10000U
EUR 4487

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 669

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 357

dNTP Set

G050 4 x 250 ul (100 mM each)
EUR 111

dNTP mixture (10mM)

DD0056 0.5ml
EUR 63.05

dNTP mixture (25mM)

DD0057 0.5ml
EUR 79.58

dNTP 10 mM

FYT013-200UL 200 µl Ask for price

dNTP Set, 100mM

BIO-39025 4 x 25µmol, (4 x 250µl) Ask for price

dNTP Set, 100mM

BIO-39026 4 x 100µmol, (4 x 4 x 250µl) Ask for price

dNTP Set, 100mM

BIO-39049 4 x 100µmol, (4 x 1ml) Ask for price

100mM dNTP Set

PR3101-S-1 1 PC
EUR 170.27

100mM dNTP Set

PR3101-S-4 1 PC
EUR 430.26

dNTP Set, 100mM each

40052 4x250uL
EUR 260
Description: Minimum order quantity: 1 unit of 4x250uL

NTP Mix

G943 250 ul 25 mM each
EUR 78

NTP Mix

G944 500 ul 25 mM each
EUR 97

NTP Mix

G945 1.0 ml 25 mM each
EUR 130

PCR Mix

L5051100 2.5 ml
EUR 107
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

dNTP Set (100mM each) - 4x0.5ml

DD0058 4x0.5ml
EUR 206.6

HiFi DNA Polymerase wi dNTP

FYT003-500U 500U Ask for price

Aflatoxin MIX Standard

SD009 0.5ug/mL
EUR 607

ACCUZYME Mix, 2x

BIO-25028 500 Reactions Ask for price

MyTaq Mix, 2x

BIO-25041 200 Reactions Ask for price

MyTaq Mix, 2x

BIO-25041/S Sample Ask for price

MyTaq Mix, 2x

BIO-25042 1000 Reactions Ask for price

MyFi Mix, 2x

BIO-25049 100 Reactions Ask for price

MyFi Mix, 2x

BIO-25049/S Sample Ask for price

MyFi Mix, 2x

BIO-25050 500 Reactions Ask for price

Ranger Mix, 2x

BIO-25051/S Sample Ask for price

Ranger Mix, 2x

BIO-25052 500 Reactions Ask for price

Powerpol 2xPCR mix

RK20718 1mL Ask for price

Individual Reaction Mix

G065-X 200 reactions
EUR 167

Jade? Master Mix

M1105-500
EUR 441

Jade? Mix-iCycler

M1106-500
EUR 441

Jade? Mix-ROX

M1108-500
EUR 441

Jade Express? Mix

M1109-500
EUR 441

Jade Smart? Mix

M1113-500
EUR 441

Jade miRNA? Mix

M1117-500
EUR 441

Taqman Master Mix

M1121-500
EUR 441

Ready? PCR Mix

M1127-1000
EUR 512

Ready? PCR Mix

M1127-200
EUR 191

Rigor? PCR Mix

M1132-200
EUR 321

Breeze? PCR Mix

M1134-200
EUR 267

Distant? PCR Mix

M1136-200
EUR 294

Advance? PCR Mix

M1139-200
EUR 376

Glucose Nutrient Mix

109 150 g
EUR 67

Green Taq Mix

P131-01 5 ml
EUR 119

Green Taq Mix

P131-02 15 ml
EUR 150

Green Taq Mix

P131-03 50 ml
EUR 248

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

dNTP Set 100 mM 4x0.4 ml

BH40203 4x0.4ml
EUR 149
Description: High purity buffer for various PCR applications.

Taq Plus DNA Polmerase (with dNTP)

P201-d1 250 U
EUR 126

Taq Plus DNA Polmerase (with dNTP)

P201-d2 1000 U
EUR 193