naclai

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Asymmetric copper-catalyzed conjugate additions of organometallic reagents in the syntheses of natural compounds and pharmaceuticals.

Access to enantiopure complex molecular structures is crucial for the development of new drugs as well as agents used in crop-protection. In this regard, numerous asymmetric methods have been established. Copper-catalyzed 1,4-additions of organometallic reagents are robust C-C bond formation strategies applicable in a wide range of circumstances.
This review analyses the syntheses of natural products and pharmaceutical agents, which rely on the application of asymmetric Cu-catalyzed conjugate additions of various organometallic reagents. A wide range of available organometallics, e.g. dialkylzinc, trialkylaluminum, Grignard, and organozirconium, can now be used in conjugate additions to address various synthetic challenges present in targeted natural compounds. Furthermore, efficient catalysts allow high levels of stereofidelity over a diverse array of starting Michael acceptors.
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Taq DNA Polymerase

BA00104 1000U
EUR 147.6
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

BA00105 2500U
EUR 278.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10101 500U
EUR 96
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10102 1000U
EUR 130.8
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA polymerase

BT10103 2000U
EUR 200.4
Description: High quality Taq polymerase for different PCR variations and downstream applications.

Taq DNA Polymerase

9001-2500 each
EUR 614.4

Taq DNA Polymerase

9001-500 each
EUR 229.2

Taq DNA Polymerase

L7051001 1000 unit
EUR 159

Taq DNA Polymerase

L7051200 200 unit
EUR 41

Taq DNA Polymerase (3000U)

9K-001-0002 3000U
EUR 582

Taq DNA Polymerase (6000U)

9K-001-0033 6000U
EUR 1234.5

Taq DNA Polymerase (1000U)

9K-001-0035 1000U
EUR 418.62

Taq DNA Polymerase (500U)

9K-001-0001 500U
EUR 209.29

Taq HS DNA Polymerase

P132-d1 500 U
EUR 38.28

Taq HS DNA Polymerase

P132-d2 1,000 U
EUR 71.34

Taq HS DNA Polymerase

P132-d3 5,000 U
EUR 324.8

Taq DNA polymerase (+dNTPs)

02-001 200U
EUR 183.6
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (+dNTPs)

02-001-5 5×200U
EUR 308.4
Description: The Taq DNA polymerase (+dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011 200U
EUR 168
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Taq DNA polymerase (-dNTPs)

02-011-5 5×200U
EUR 261.6
Description: The Taq DNA polymerase (-dNTPs) is available in Europe and for worldwide shipping via Gentaur.

Maximo Taq DNA Polymerase

S101 500 units
EUR 55.2

Maximo Taq DNA Polymerase

S102 5x500 units
EUR 128.4

Maximo Taq DNA Polymerase

S103 20x500 units
EUR 393.6

Taq DNA Polymerase, 5units/㎕

T2200-050 500units
EUR 157.2

Taq DNA Polymerase, 5units/㎕

T2200-100 2x500 units
EUR 230.4

Taq DNA Polymerase, 5units/㎕

T2200-200 4x500 units
EUR 375.6

Hotstart Taq DNA Polymerase

L7052001 1000 units
EUR 567

Hotstart Taq DNA Polymerase

L7052250 250 units
EUR 159

Champagne Taq DNA Polymerase

P122-d1 500 U (2.5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d2 500 U (5 U/μl)
EUR 212.4

Champagne Taq DNA Polymerase

P122-d3 500 U (10 U/μl)
EUR 212.4

ACTaq? Taq DNA Polymerase, 1000U

E2100-1000U each
EUR 260.4

Taq DNA Polymerase - 5,000 units

3245 1/EA
EUR 620.4

Recombinant Taq Dna Polymerase

27079804 EACH
EUR 117.6

Recombinant Taq Dna Polymerase

27079806 EACH
EUR 639.6

Taq DNA Polymerase (Recombinant)

abx073521-10000U 10000 U
EUR 710.4

Taq DNA Polymerase (Recombinant)

abx073521-1000U 1000 U
EUR 276

Taq DNA Polymerase (Recombinant)

abx073521-3000U 3000 U
EUR 393.6

Recombinant Taq DNA Polymerase

7-03634 1,000U Ask for price

Recombinant Taq DNA Polymerase

7-03635 3,000U Ask for price

Recombinant Taq DNA Polymerase

7-03636 10,000U Ask for price

ACTaq? Taq DNA Polymerase, 250U

E2100-250U each
EUR 122.4

Taq Plus DNA Polymerase

P201-01 250 U
EUR 147.6

Taq Plus DNA Polymerase

P201-02 1000 U
EUR 213.6

Taq Plus DNA Polymerase

P201-03 3000 U
EUR 382.8

Taq DNA Polymerase, 5u/ul

B0089(D0089) 5X200U
EUR 112.2

TaqFast DNA Polymerase (PCR)

G277 250 U (50 ul)
EUR 87.6

TaqFast DNA Polymerase (PCR)

G278 1000 U (200 ul)
EUR 160.8

Genorise® Taq DNA Polymerase

GR108007 200 tests
EUR 230

YEA taq DNA Polymerase wiodNTP

FYT011-500U 500U Ask for price

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S400 200 units
EUR 86.4

Maximo H-SPlus Taq DNA Polymerase (for Hot-Start PCR)

S410 1000 units
EUR 231.6

Recombinant (E.Coli) Taq DNA Polymerase

RP-394 1000 IU
EUR 196.8

Taq DNA Polymerase Polyclonal Antibody

7018-100 each
EUR 424.8

Taq DNA Polymerase Polyclonal Antibody

7018-30T each
EUR 175.2

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ001 1000U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ100 100U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ200 200U Ask for price

Taq DNA Polymerase, 5 units/µl

ABP-PP-ERTQ500 500U Ask for price

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

ACTaq? Taq Blue DNA Polymerase, 1000U

E2100B-1000U each
EUR 272.4

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ plus Buffer)

P101-03 10000 U
EUR 314.4

Taq DNA Polymerase (Mg2+ free Buffer)

P102-01 1000 U
EUR 145.2

Taq DNA Polymerase (Mg2+ free Buffer)

P102-02 5000 U
EUR 231.6

Taq DNA Polymerase (Mg2+ free Buffer)

P102-03 10000 U
EUR 314.4

ACTaq? Taq Blue DNA Polymerase, 250U

E2100B-250U each
EUR 126

Taq DNA polymerase (TQ21) , DNA Aptamer, unlabeled

AD-146-U Custom Ask for price

Cheetah Hot Start Taq DNA Polymerase

29050 500EU
EUR 355.2
Description: Minimum order quantity: 1 unit of 500EU

Taq Plus DNA Polymerase, 5u/ul

D0090 200U
EUR 94.8

Taq Plus DNA Polymerase (Recombinant)

abx073522-2500U 2.500 U
EUR 710.4

Taq Plus DNA Polymerase (Recombinant)

abx073522-250U 250 U
EUR 276

Taq Plus DNA Polymerase (Recombinant)

abx073522-750U 750 U
EUR 393.6

Recombinant Taq Plus DNA Polymerase

7-03631 250U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03632 750U Ask for price

Recombinant Taq Plus DNA Polymerase

7-03633 2,500U Ask for price

Taq HS DNA Polymerase (Glycerol-free)

QL101-01 1,000 U
EUR 71.2

Taq HS DNA Polymerase (Glycerol-free)

QL101-02 5,000 U
EUR 324.8

Evaluation of a new thromboplastin reagent STA-NeoPTimal on a STA R Max analyzer for the measurement of prothrombin time, international normalized ratio and extrinsic factor levels

Introduction: We aimed at evaluating the performance of a new prothrombin time (PT) reagent (STA-NeoPTimal) with two other PT reagents (STA-Neoplastine R and STA-Neoplastine CI Plus) and the reference PT reagent used in our laboratory (ReadiPlasTin).
Methods: Evaluation consisted in intra- and interassay precision assessment, determination of sensitivity to unfractionated heparin (UFH) or enoxaparin in spiked samples and to direct oral anticoagulants (DOACs) in patients (n = 43). Method comparison of the 4 PT reagents, factor II, V, VII and X assays was tested on normal (n = 20) and abnormal samples: VKA (n = 47), preoperative (n = 23), liver failure (n = 12) and burned patients (n = 37).
Results: Analytical performance met manufacturers’ criteria for all reagents. All PT reagents gave correlation coefficients >0.8 and even >0.9 in many situations.
In some VKA samples, differences ≥ 0.5 INR units were found in samples within and above therapeutic ranges. For burned patients, PT correlations were good but with some minimal bias (<5.0%) while factor assays gave very consistent results (R > .8 and mainly >0.9). As expected, poor responsiveness of the PT to DOAC concentrations was observed with all four assays.
Conclusion: The STA-NeoPTimal showed comparable performance to ReadiPlasTin, making it suitable for VKA control, detection of factors II, V, VII, X deficiency and assessment of liver disease coagulopathy.
However, for patients receiving VKA, some significant differences were observed. We confirmed the inability of the PT assay to detect residual DOAC concentrations. Finally, burned patients results showed that recombinant thromboplastins were less sensitive to factor deficiencies in comparison to extraction thromboplastins.

An Experimental and Theoretical Study on the “2,2′ Bipiridyl Ni Catalyzed” Hirao Reaction of >P(O)H Reagents and Halobenzenes; A Ni(0)→Ni(II) or a Ni(II)→Ni(IV) mechanism?

The P-C couplings between >P(O)H reagents and halogenoarenes may take place either by Pd- or Ni-catalysis. However, the latter version has been less studied. It was found by us that the reaction of Ph2P(O)H or PhEtOP(O)H with PhX (X = I and Br) in the presence of NiCl2/Zn as the precursors for the assumed Ni(0) complexant together with 2,2′-bipyridine as the ligand took place only with PhI at 50/70 °C. M06-2X/6-31G(d,p)//PCM(MeCN) calculations for the P-C coupling of Ph2P(O)H and PhX revealed a favorable energetics only for the loss of iodide following the oxidative addition of PhI on the Ni(0) atom.
  • However, the assumed transition states with Ni(II) formed after P-ligand uptake and deprotonation could not undergo reductive elimination meaning a “dead-end street” in the mechanism expected. Hence, it was assumed that the initial complexation of the remaining Ni2+ ions with 2,2′-bipyridine may move the P-C coupling forward via a Ni(II)→Ni(IV) transition.
  • This route was also evaluated by calculations, and the mechanism explored was justified by preparative experiments carried out using NiCl2 and bipyridine, but in the absence of Zn. There was no need for Zn, when bipyridine was applied as the N-ligand, moreover PhBr could also be a suitable starting material at higher temperatures.
  • Hence, the generally accepted Ni(0)→Ni(II) route was refuted by us, confirming, surprisingly, the generality of the Ni(II)→N(IV) protocol, either in the presence of bipyridine, or using the excess of the >P(O)H ⇌ >P-OH reagent as the P-ligand.
  • The results of the calculations on the complex forming ability of Ni(0) and Ni(II) with 2,2′-bipyridine or the tautomeric >P-OH form of the P-reagents were in accord with our mechanistic proposition. Our study also means the clarification of the contradicting and sometimes uncertain literature data published in the last decade.

dNTP MIX 100mM, 1ml

2013MIX-1ML each
EUR 184.8

dNTP/dUTP Mix

DB2236 1ml
EUR 70.44

dNTP Mix (1 ml)

9002-1 each
EUR 183.6

dNTP Mix, 100mM Final Conc.

BIO-39028 50µmol, (1 x 500µl) Ask for price

dNTP Mix, 100mM Final Conc.

BIO-39029 200µmol, (4 x 500µl) Ask for price

dNTP Mix (10 mM each)

P031-01 1 ml
EUR 142.8

dNTP Mix (10 mM each)

P031-02 5 ml
EUR 213.6

dNTP Mix (2.5 mM each)

P032-01 1 ml
EUR 129.6

dNTP Mix (2.5 mM each)

P032-02 5 ml
EUR 162

dNTP Mix, 40mM Final Conc.

BIO-39043 20µmol, (1 x 500µl) Ask for price

dNTP Mix, 10mM Final Conc.

BIO-39044 10µmol, (1ml vol.) Ask for price

dNTP Mix, 10mM Final Conc.

BIO-39053 100µmol, (10 x 1ml) Ask for price

ReadiUseâ„¢ dNTP Mix *10 mM*

17200-5mL 5 mL
EUR 318
Description: Our Ready-to Use Solution of deoxynucleotide triphosphates (dNTPs) have been extensively tested and verified for use in a wide variety of molecular biology applications, including PCR, real-time PCR, high-fidelity PCR, long PCR, cDNA synthesis, RT-PCR, RCA, MDA, DNA labeling, DNA sequencing, in vitro transcription, siRNA synthesis, and RNA amplification.

High Quality dNTP Mix, 10mM

ABP-PP-ERDN100 1mL Ask for price

High Quality dNTP Mix, 10mM

ABP-PP-ERDN500 5mL Ask for price

dNTP Mix (10 mM each) 1ml

9K-003-0006 1ml
EUR 174.84

dNTP Mix (10 mM each) 5x1ml

9K-003-0013 5x1mL
EUR 615.06

ReadiUse™ dNTP Mix *10 mM*

17200 5 mL
EUR 318

Taq DNA Polymerase with dNTP Mix (6000U)

9K-001-0018 6000U
EUR 1536.74

Taq DNA Polymerase with dNTP Mix (3000U)

9K-001-0032 3000U
EUR 1088.87

Taq DNA Polymerase with dNTP Mix (1000U)

9K-001-0034 1000U
EUR 518.84

Fast-Pfu with 10mM dNTP Mix (250U)

9K-001-0022 250U
EUR 482.82

Fast-Pfu with 10mM dNTP Mix (500U)

9K-001-0023 500U
EUR 741.22

Taq DNA Polymerase with dNTP Mix (500U)

9K-001-0031 500U
EUR 351.28

40mM dNTP Mix (10mM/80µm each), 2ml

PR3040-M-4 1 PC
EUR 153

40mM dNTP Mix (10mM/20µmol each), 0.5ml

PR3040-M-1 1 PC
EUR 41.2

LR DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0008 250U
EUR 404.52

Pfu DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0009 500U
EUR 686.4

Pfu DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0010 250U
EUR 396.7

Fast-Taq DNA Polymerase with 10mM dNTP Mix (2500U)

9K-001-0004 2500U
EUR 2465.38

High-Taq DNA Polymerase with 10mM dNTP Mix (5000U)

9K-001-0020 5000U
EUR 2878.8

Pfu DNA Polymerase with 10mM dNTP Mix (10, 000U)

9K-001-0021 10000U
EUR 5384.4

ReadiUseâ„¢ dNTP Mix Set *10 mM PCR Grade*

17258-1mL 1 mL
EUR 79
Description: 10 mM dNTP Mix is a mixture of four nucleotides (dATP, dCTP, dGTP, dTTP) in purified water.

Fast-Taq DNA Polymerase with 10mM dNTP Mix (500U)

9K-001-0003 500U
EUR 575.22

High-Taq DNA Polymerase with 10mM dNTP Mix (250U)

9K-001-0005 250U
EUR 384.17

ReadiUse™ dNTP Mix Set *10 mM PCR Grade*

17258 1 mL
EUR 79

Taq DNA Polymerase - 5,000 units with separate dNTP Mix

3245d 1/EA
EUR 802.8

High-Taq DNA Polymerase with 10mM dNTP Mix (4x250U)

9K-001-0006 4x250U
EUR 1055.98

dNTP mixture (10mM)

DD0056 0.5ml
EUR 75.66

dNTP mixture (25mM)

DD0057 0.5ml
EUR 95.5

Hot Start Taq DNA Polymerase - 1000 units with seperate dNTP mix

3293d 1/EA
EUR 428.4

dNTP Set

G050 4 x 250 ul (100 mM each)
EUR 133.2

dNTP Mixtures ,10mM each , >99% by HPLC

D0610-050 500ul
EUR 109.2

dNTP Mixtures ,10mM each , >99% by HPLC

D0610-100 10ml
EUR 475.2

dNTP Mixtures ,10mM each , >99% by HPLC

D0610-105 10x500ul
EUR 343.2

dNTP Mixtures ,10mM each , >99% by HPLC

D0610-205 20x500ul
EUR 537.6

dNTP Mixtures ,10mM each , >99% by HPLC

D0610-500 50ml
EUR 2020.8

dNTP 10 mM

FYT013-200UL 200 µl Ask for price

dNTP Set, 100mM

BIO-39025 4 x 25µmol, (4 x 250µl) Ask for price

dNTP Set, 100mM

BIO-39026 4 x 100µmol, (4 x 4 x 250µl) Ask for price

dNTP Set, 100mM

BIO-39049 4 x 100µmol, (4 x 1ml) Ask for price

dNTP Set (100mM each) - 4x0.5ml

DD0058 4x0.5ml
EUR 247.92

HiFi DNA Polymerase wi dNTP

FYT003-500U 500U Ask for price

dNTP Set 100 mM 4x0.4 ml

BH40203 4x0.4ml
EUR 178.8
Description: High purity buffer for various PCR applications.

YEA taq DNA Polymerase wi dNTP

FYT001-500U 500U Ask for price

Dntp Set - A C G T 500Ul

28406560 EACH
EUR 194.4

Dntp Set - A C G T 500Ul

28406564 EACH
EUR 115.2

Taq Plus DNA Polmerase (with dNTP)

P201-d1 250 U
EUR 151.2

Taq Plus DNA Polmerase (with dNTP)

P201-d2 1000 U
EUR 231.6

Taq Plus DNA Polmerase (with dNTP)

P201-d3 3000 U
EUR 424.8

Dntp Set 100 Mm 4 x 25Umol

28406551 EACH
EUR 226.8

Dntp Set 100 Mm 4 x 100Umol

28406552 EACH
EUR 673.2

dNTP set (each 100mM) (4 x 1mL)

9K-003-0007 4x1mL, 4ml
EUR 726.42

Dntp Set - A C G T 1x10Umol

28406557 EACH
EUR 138

Dntp Set - A C G T 4x10Umol

28406558 EACH
EUR 333.6

Dntp Set - A C G T 4x500Umol

28406553 EACH
EUR 2468.4

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-010 100 units
EUR 180

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl3

P0330-020 2x100 units
EUR 286.8

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-050 5X100 units
EUR 465.6

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-250 25X100 units
EUR 2004

amfiXpand Taq DNA Polymerase,Including dNTP&MgCl2

P0330-500 50X100 units
EUR 3591.6

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-020 200 units
EUR 313.2

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-040 2X200 units
EUR 498

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-120 6x200 units
EUR 1178.4

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-400 20x200 units
EUR 2895.6

amfiXpand Hot-Start Version , Including dNTP&MgCl2

P0334-800 40x200 units
EUR 5406

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ plus Buffer, with dNTP)

P101-d3 10000 U
EUR 490.8

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d1 1000 U
EUR 163.2

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d2 5000 U
EUR 320.4

Taq DNA Polymerase (Mg2+ free Buffer, with dNTP)

P102-d3 10000 U
EUR 490.8

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d1 125 U
EUR 147.6

Vazyme LAmp DNA Polymerase (Mg2+ plus buffer, with dNTP)

P301-d2 500 U
EUR 213.6

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)

P302-d1 125 U
EUR 147.6

Vazyme LAmp DNA Polymerase (Mg2+ free buffer, with dNTP)

P302-d2 500 U
EUR 213.6