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August 26, 2020

High density lipoprotein phospholipid using a sensitive reagent 

By Brandy

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Enzymatic determination of high density lipoprotein phospholipid using a sensitive reagent and a Cobas Bio Centrifugal Analyzer.

A sensitive enzymatic method for the measurement of high density lipoprotein choline-containing phospholipids (HDL-pc) was adapted to a Cobas Bio Centrifugal Analyzer. The Boehringer Mannheim phospholipase D/choline oxidase/peroxidase reagent was modified by the inclusion of 2,4,6,-tribromo-3-hydroxybenzoic acid (TBHBA) which reacts with hydrogen peroxide and 4-aminophenazone to produce a quinone-imine dye with a fourfold greater molar absorption than that produced with phenol.
The method has been developed for the determination of HDL fractions isolated with polyethylene glycol 6000, for which a reagent of high sensitivity is required. The method is linear to 5.5 mmol/l of HDL-pc and the CVs for within-run and day-to-day imprecision were less than 3.5%. Correlation of HDL cholesterol (HDL-c) with HDL-pc values in 200 healthy subjects was good (r = 0.9024). The mean (+/- SD) HDL-pc value for men was 1.12 (+/- 0.26) and for women, 1.34 (+/- 0.29) mmol/l. The assay is inexpensive and large numbers of specimens can be processed rapidly and conveniently.
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Human 11 beta hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA Kit
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Human 11 beta hydroxysteroid dehydrogenase type 1 (11β-HSD1) ELISA Kit
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Human 11 beta hydroxysteroid dehydrogenase type 1 (11beta HSD1) ELISA Kit
MBS7238715-10x96StripWells 10x96-Strip-Wells
EUR 5685

Human 11 beta hydroxysteroid dehydrogenase type 1 (11beta HSD1) ELISA Kit
MBS7238715-48StripWells 48-Strip-Wells
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Human 11 beta hydroxysteroid dehydrogenase type 1 (11beta HSD1) ELISA Kit
MBS7238715-5x96StripWells 5x96-Strip-Wells
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Human 11 beta hydroxysteroid dehydrogenase type 1 (11beta HSD1) ELISA Kit
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Rat 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
MBS726807-10x96StripWells 10x96-Strip-Wells
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Rat 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
MBS726807-48StripWells 48-Strip-Wells
EUR 485

Rat 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
MBS726807-5x96StripWells 5x96-Strip-Wells
EUR 3020

Rat 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Goat 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Goat 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Goat 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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EUR 3020

Goat 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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EUR 690

Sheep 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
MBS7204552-10x96StripWells 10x96-Strip-Wells
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Sheep 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Sheep 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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EUR 690

Canine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
MBS7208040-10x96StripWells 10x96-Strip-Wells
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Canine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Canine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Canine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Monkey 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Monkey 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Monkey 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Monkey 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Bovine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Bovine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Bovine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Bovine 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Rabbit 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Rabbit 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Rabbit 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Rabbit 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Mouse 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Mouse 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Mouse 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Human 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Human 11 beta hydroxysteroid dehydrogenase type 1 (11beta-HSD1) ELISA Kit
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Dog 3 beta hydroxysteroid dehydrogenase type 1 ELISA kit
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EUR 624
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EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine 3 beta hydroxysteroid dehydrogenase type 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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EUR 624
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EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine 17 beta hydroxysteroid dehydrogenase type 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

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EUR 700
Description: ELISA

Rat 17 beta hydroxysteroid dehydrogenase type 1 ELISA kit
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EUR 700
Description: ELISA

Goat 3 beta hydroxysteroid dehydrogenase type 1 ELISA kit
E01A45705 96T
EUR 700
Description: ELISA

Goat 17 beta hydroxysteroid dehydrogenase type 1 ELISA kit
E01A45706 96T
EUR 700
Description: ELISA

Goat 3 beta hydroxysteroid dehydrogenase type 1 ELISA kit
E06B0996-192T 192 tests
EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat 3 beta hydroxysteroid dehydrogenase type 1 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Optimization of EMIT reagent system using a COBAS-BIO centrifugal analyzer.

An optimized method for application of the EMIT reagent system to a COBAS-BIO centrifugal analyzer was developed to minimize reagent consumption while maintaining good precision and accuracy. Forty-one serum samples, previously analyzed for theophylline by HPLC, were assayed using the modified protocol on the COBAS-BIO centrifugal analyzer. Regression analysis of the results obtained from both methods yielded the following equation: y = 1.10x + 0.01, r = 0.964 (x = HPLC, y = COBAS-BIO centrifugal analyzer, EMIT). The COBAS-BIO centrifugal analyzer and EMIT methodology provided an efficient, inexpensive, and relatively effortless approach to drug monitoring in the routine clinical laboratory.

Adaptation of EMIT reagents to the Cobas Bio centrifugal analyzer.

Our adaptation of EMIT enzyme immunoassay reagents to the Cobas Bio centrifugal analyzer avoids the decrease in precision at the upper end of the therapeutic range reported in other adaptations. Long-term (12-month) CVs for lidocaine, valproic acid, and tobramycin were 5% or less; for quinidine, acetaminophen, and phenytoin, long-term CVs ranged from 7.2% to 8.6%.

A rapid and precise method for the quantitation of ethanol in serum using the EMIT St serum ethanol assay reagent and a Cobas Bio centrifugal analyser.

We describe a simple and rapid, but nevertheless precise and accurate method for measuring ethanol in serum, using the Roche Cobas Bio centrifugal analyser and the EMIT StTM serum ethanol assay reagent. Within-assay imprecision was 2.2% (CV), day-to-day imprecision was 2.5% (CV). The results correlated well with a commonly used gas chromatographic headspace method. Because of its rapidity and good practicability this method seems to be well suited for use in emergency care units of clinical chemistry laboratories.

Reconfigurable Flow Platform for Automated Reagent Screening and Autonomous Optimization for Bio-inspired Lignans Synthesis.

Naturally occurring benzoxanthenones which belongs to the vast family of lignans, are promising biologically relevant targets. They are biosynthetically produced by the oxidative dimerization of 2-propenyl phenols. In this manuscript, we disclose a powerful automated flow-based strategy for identifying and optimizing a cobalt-catalyzed oxidizing system for the bio-inspired dimerization of 2-propenyl phenols.
  • We designed a reconfigurable flow reactor associating on-line monitoring and process-control instrumentation. Our machine was first configured as an automated screening platform to evaluate a matrix of 4 catalysts (plus the blank) and 5 oxidant (plus the blank) at two different temperatures, resulting in an array of 50 reactions.
  • The automated screening was conducted on micromole scale at a rate of one fully characterized reaction every 26 minutes. After having identified the most promising cobalt-catalyzed oxidizing system, the automated screening platform was straightforwardly reconfigured to an autonomous self-optimizing flow reactor by implementation of an optimization algorithm in the closed-loop system.
  • The optimization campaign allowed the determination of very effective experimental conditions in a limited number of experiments which allowed to prepare natural products carpanone and polemannone B as well as synthetic analogues.

2,3-dinor-TXB2 (2,3-dinor Thromboxane B2) ELISA Kit
MBS2511399-10x96Tests 10x96Tests
EUR 3265

2,3-dinor-TXB2 (2,3-dinor Thromboxane B2) ELISA Kit
MBS2511399-24Tests 24Tests
EUR 225

2,3-dinor-TXB2 (2,3-dinor Thromboxane B2) ELISA Kit
MBS2511399-48Tests 48Tests
EUR 350

2,3-dinor-TXB2 (2,3-dinor Thromboxane B2) ELISA Kit
MBS2511399-5x96Tests 5x96Tests
EUR 1655

2,3-dinor-TXB2 (2,3-dinor Thromboxane B2) ELISA Kit
MBS2511399-96Tests 96Tests
EUR 395

Human 2,3-dinor thromboxane B2(2,3-dinor-TXB2)ELISA Kit
GA-E1149HM-48Tests 48 Tests Ask for price

Human 2,3-dinor thromboxane B2(2,3-dinor-TXB2)ELISA Kit
GA-E1149HM-96Tests 96 Tests
EUR 388

Human 2,3-dinor thromboxane B2(2,3-dinor-TXB2)ELISA Kit
GA-E1149HM-48T 48T
EUR 346.8

Human 2,3-dinor thromboxane B2(2,3-dinor-TXB2)ELISA Kit
GA-E1149HM-96T 96T
EUR 559.2

Human 2,3-dinor thromboxane B2(2,3-dinor-TXB2)ELISA Kit
QY-E05242 96T
EUR 433.2

2,3-dinor-TXB2 ELISA Kit| General 2,3-dinor Thromboxane B2 ELISA
EF019592 96tests
EUR 566

2,3-dinor Thromboxane B2 (2,3-dinor-TXB2) ELISA Kit, 96 tests, Quantitative
10875 1 Kit
EUR 927.6

Human 2, 3-dinor thromboxane B2, 2, 3-dinor-TXB2 ELISA Kit
MBS9303770-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human 2, 3-dinor thromboxane B2, 2, 3-dinor-TXB2 ELISA Kit
MBS9303770-48StripWells 48-Strip-Wells
EUR 550

Human 2, 3-dinor thromboxane B2, 2, 3-dinor-TXB2 ELISA Kit
MBS9303770-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human 2, 3-dinor thromboxane B2, 2, 3-dinor-TXB2 ELISA Kit
MBS9303770-96StripWells 96-Strip-Wells
EUR 765

Human 2, 3-dinor thromboxane B2 (2, 3-dinor-TXB2) ELISA Kit
MBS9312577-10x96StripWells 10x96-Strip-Wells
EUR 6725

Human 2, 3-dinor thromboxane B2 (2, 3-dinor-TXB2) ELISA Kit
MBS9312577-48StripWells 48-Strip-Wells
EUR 550

Human 2, 3-dinor thromboxane B2 (2, 3-dinor-TXB2) ELISA Kit
MBS9312577-5x96StripWells 5x96-Strip-Wells
EUR 3420

Human 2, 3-dinor thromboxane B2 (2, 3-dinor-TXB2) ELISA Kit
MBS9312577-96StripWells 96-Strip-Wells
EUR 765

2,3-dinor Thromboxane B1
T36220-10mg 10mg Ask for price
Description: 2,3-dinor Thromboxane B1

2,3-dinor Thromboxane B1
T36220-1g 1g Ask for price
Description: 2,3-dinor Thromboxane B1

2,3-dinor Thromboxane B1
T36220-1mg 1mg Ask for price
Description: 2,3-dinor Thromboxane B1

2,3-dinor Thromboxane B1
T36220-50mg 50mg Ask for price
Description: 2,3-dinor Thromboxane B1

2,3-dinor Thromboxane B1
T36220-5mg 5mg Ask for price
Description: 2,3-dinor Thromboxane B1

2,3-dinor Thromboxane B1
MBS5797401-INQUIRE INQUIRE Ask for price

Human 2,3 dinor tromboxan B2, 2,3 dinor TXB2 ELISA Kit
MBS1609975-10x96StripWells 10x96-Strip-Wells
EUR 3460

Human 2,3 dinor tromboxan B2, 2,3 dinor TXB2 ELISA Kit
MBS1609975-5x96StripWells 5x96-Strip-Wells
EUR 1750

Human 2,3 dinor tromboxan B2, 2,3 dinor TXB2 ELISA Kit
MBS1609975-96StripWells 96-Strip-Wells
EUR 425

Rat 2.3 dinor-thromboxane B2(2,3-dinor-TXB2)ELISA Kit
NSL1625r 96 Tests
EUR 498

Rat 2.3 dinor-thromboxane B2(2,3-dinor-TXB2) ELISA Kit
EIA05159r each
EUR 480

Thromboxane B2 (TXB2) ELISA Kit
20-abx150379
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  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Thromboxane B2 (TXB2) ELISA Kit
abx255576-96tests 96 tests
EUR 801.6

Thromboxane B2 (TXB2) ELISA Kit
abx575839-96tests 96 tests
EUR 801.6

Thromboxane B2 (TXB2) ELISA Kit
abx364664-96tests 96 tests
EUR 1111.2

Thromboxane B2 (TXB2) ELISA Kit
abx358004-96tests 96 tests
EUR 990

Thromboxane B2 (TXB2) ELISA Kit
abx357043-96tests 96 tests
EUR 990

Thromboxane B2 (TXB2) ELISA Kit
DLR-TXB2-Ge-48T 48T
EUR 562.8
Description: A competitive inhibition quantitative ELISA assay kit for detection of Thromboxane B2 (TXB2) in samples from serum, plasma or other biological fluids.

Thromboxane B2 (TXB2) ELISA Kit
DLR-TXB2-Ge-96T 96T
EUR 729.6
Description: A competitive inhibition quantitative ELISA assay kit for detection of Thromboxane B2 (TXB2) in samples from serum, plasma or other biological fluids.

Thromboxane B2 (TXB2) ELISA Kit
EKN48761-48T 48T
EUR 430.43

Thromboxane B2 (TXB2) ELISA Kit
EKN48761-5x96T 5x96T
EUR 2920.78

Thromboxane B2 (TXB2) ELISA Kit
EKN48761-96T 96T
EUR 614.9

Thromboxane B2 (TXB2) ELISA Kit
EKU10375-48T 48T
EUR 948.01

Thromboxane B2 (TXB2) ELISA Kit
EKU10375-5x96T 5x96T
EUR 6432.93

Thromboxane B2 (TXB2) ELISA Kit
EKU10375-96T 96T
EUR 1354.3

Thromboxane B2 (TXB2) ELISA Kit
EKN51486-48T 48T
EUR 589.75

Thromboxane B2 (TXB2) ELISA Kit
EKN51486-5x96T 5x96T
EUR 4001.88

Thromboxane B2 (TXB2) ELISA Kit
EKN51486-96T 96T
EUR 842.5

Thromboxane B2 (TXB2) ELISA Kit
EKU07650-48T 48T
EUR 632.03

Thromboxane B2 (TXB2) ELISA Kit
EKU07650-5x96T 5x96T
EUR 4288.78

Thromboxane B2 (TXB2) ELISA Kit
EKU07650-96T 96T
EUR 902.9

Thromboxane B2 (TXB2) ELISA Kit
abx257167-05mg 0.5 mg Ask for price

Thromboxane B2 (TXB2) ELISA Kit
abx257167-100g 100 µg
EUR 525

Thromboxane B2 (TXB2) ELISA Kit
abx257167-1mg 1 mg Ask for price

Human 2, 3 dinor tromboxan B2, 2, 3 dinor TXB2 GENLISA ELISA
KBH4168 1 x 96 wells
EUR 286

TXB2(Thromboxane B2) ELISA Kit
EKF60164-48T 48T
EUR 396.9

TXB2(Thromboxane B2) ELISA Kit
EKF60164-5x96T 5x96T
EUR 2693.25

TXB2(Thromboxane B2) ELISA Kit
EKF60164-96T 96T
EUR 567

TXB2(Thromboxane B2) ELISA Kit
ELK7860-48T 48T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TXB2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TXB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TXB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

TXB2(Thromboxane B2) ELISA Kit
ELK7860-96T 96T Ask for price
Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TXB2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TXB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TXB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

TXB2 (Thromboxane B2) ELISA Kit
MBS7608060-10x96StripWells 10x96-Strip-Wells
EUR 3900

TXB2 (Thromboxane B2) ELISA Kit
MBS7608060-48StripWells 48-Strip-Wells
EUR 340

TXB2 (Thromboxane B2) ELISA Kit
MBS7608060-5x96StripWells 5x96-Strip-Wells
EUR 2045

TXB2 (Thromboxane B2) ELISA Kit
MBS7608060-96StripWells 96-Strip-Wells
EUR 455

Rat Thromboxane B2(TXB2) Elisa Kit
EK720869 96 Wells
EUR 0.61

Rat Thromboxane B2,TXB2 ELISA Kit
EKC39767-48T 48T
EUR 439.25

Rat Thromboxane B2,TXB2 ELISA Kit
EKC39767-5x96T 5x96T
EUR 2980.63

Rat Thromboxane B2,TXB2 ELISA Kit
EKC39767-96T 96T
EUR 627.5

Rat Thromboxane B2, TXB2 ELISA Kit
CSB-E08047r-24T 1 plate of 24 wells
EUR 198
Description: Quantitativesandwich ELISA kit for measuring Rat Thromboxane B2, TXB2 in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Rat Thromboxane B2, TXB2 ELISA Kit
1-CSB-E08047r
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  • Ask for price
  • Ask for price
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Rat Thromboxane B2, TXB2 in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Rat Thromboxane B2,TXB2 ELISA Kit
CN-01922R1 96T
EUR 530.4

Rat Thromboxane B2,TXB2 ELISA Kit
CN-01922R2 48T
EUR 351.6

Cat Thromboxane B2 (TXB2) ELISA Kit
YLA0045CT-48T 48T Ask for price

Cat Thromboxane B2 (TXB2) ELISA Kit
YLA0045CT-96T 96T Ask for price

Rat Thromboxane B2,TXB2 ELISA Kit
YLA0462RA-48T 48T Ask for price

Rat Thromboxane B2,TXB2 ELISA Kit
YLA0462RA-96T 96T Ask for price

Rat Thromboxane B2(TXB2)ELISA Kit
GA-E0692RT-48Tests 48 Tests Ask for price

Rat Thromboxane B2(TXB2)ELISA Kit
GA-E0692RT-96Tests 96 Tests
EUR 420

Rat Thromboxane B2,TXB2 ELISA KIT
E0684Ra-1096T 10*96T
EUR 4122

Rat Thromboxane B2,TXB2 ELISA KIT
E0684Ra-48wells 48 wells
EUR 300

Rat Thromboxane B2,TXB2 ELISA KIT
E0684Ra-596T 5*96T
EUR 2061

Rat Thromboxane B2,TXB2 ELISA KIT
E0684Ra-96wells 96 wells
EUR 458

Rat Thromboxane B2(TXB2)ELISA Kit
GA-E0692RT-48T 48T
EUR 380.4

Rat Thromboxane B2(TXB2)ELISA Kit
GA-E0692RT-96T 96T
EUR 595.2

Rat Thromboxane B2 (TXB2) ELISA Kit
NSL1104r 96 Tests
EUR 498

Rat Thromboxane B2(TXB2)ELISA Kit
QY-E10960 96T
EUR 433.2

Rat Thromboxane B2,TXB2 ELISA Kit
SL0678Ra -
EUR 498

Rat Thromboxane B2,TXB2 ELISA Kit
CELI-66069r 96tests
EUR 756

Rat Thromboxane B2, TXB2 ELISA Kit
MBS705104-10x96StripWells 10x96-Strip-Wells
EUR 5140
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