Tag: gelsosomo’s

August 26, 2020

High density lipoprotein phospholipid using a sensitive reagent 

Enzymatic determination of high density lipoprotein phospholipid using a sensitive reagent and a Cobas Bio Centrifugal Analyzer. A sensitive enzymatic method for the measurement of high density lipoprotein choline-containing phospholipids (HDL-pc) was adapted to a Cobas Bio Centrifugal Analyzer. The Boehringer Mannheim phospholipase D/choline oxidase/peroxidase reagent was modified by the inclusion of 2,4,6,-tribromo-3-hydroxybenzoic acid (TBHBA) which reacts with hydrogen peroxide and 4-aminophenazone to produce a quinone-imine dye with […]